Vibrio litoralis sp. nov., isolated from a Yellow Sea tidal flat in Korea

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 3; pp. 562 - 565
• Main Authors: Nam, Young-Do, Chang, Ho-Won, Park, Ja Ryeong, Kwon, Hyuk-Yong, Quan, Zhe-Xue, Park, Yong-Ha, Kim, Byung-Chun, Bae, Jin-Woo
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.03.2007; Society for General Microbiology
• Subjects: Animal, plant and microbial ecology; Bacteriology; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Korea; Microbial ecology; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Systematics; Various environments (extraatmospheric space, air, water); Vibrio; Vibrio - classification; Vibrio - genetics; Vibrio - isolation & purification; Vibrio - physiology; Vibrio litoralis; Vibrio rumoiensis; Asia; Yellow Sea; Korea; INW, Huanghai Sea; INW, Korea, Rep; Marine environment; Tidal flat; Taxonomy; Bacteria; Vibrionaceae; New species; Vibrio
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64653-0

1 Biological Resource Center, KRIBB, 52 Oeundong, Yusong, Daejeon 305-333, Korea 2 University of Science and Technology, 52, Eoeun-dong, Yuseong-gu, Daejeon 305-333, Korea 3 Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China 4 Department of Applied Microbiology, Yeungnam University, 214-1 Dae-dong Gyeongsan-si, Gyeongsangbuk-do 712-749, Korea 5 Environmental Biotechnology National Core Research Center, Kyungsang National University, Jinju, Korea Correspondence Jin-Woo Bae baejw{at}kribb.re.kr Two Gram-negative, facultatively anaerobic bacterial strains, MANO22D T and MANO22P, were isolated from a tidal flat area of Dae-Chun, Chung-Nam, Korea. The isolates were rod-shaped and were motile by means of one or more polar flagella. They grew at 1–12 % NaCl, 4–45 °C and pH 4.1–8.8 and were oxidase-positive, arginine dihydrolase-negative and sensitive to the vibriostatic agent O/129. The isolates required Na + for growth, produced acid, but no gas, from D -glucose under anaerobic conditions and utilized a wide range of compounds as sole carbon and energy sources. A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains belong to the Gammaproteobacteria and are specifically related to Vibrio species. They were most closely related to Vibrio rumoiensis FERM P-14531 T , with which they were found to share 98.65 % 16S rRNA gene sequence similarity. In the phylogenetic tree, the two novel strains comprised a relatively long subline of descent, sharing a branching point with the outlying species V. rumoiensis , and were found to occupy a phylogenetically distant position on the main Vibrio branch. The levels of DNA–DNA hybridization with respect to V. rumoiensis FERM P-14531 T , which is their most closely related phylogenetically related Vibrio species, were 7.4 % (MANO22D T ) and 3.9 % (MANO22P). Thus, the two novel isolates appear to represent a novel species within the genus Vibrio , for which the name Vibrio litoralis sp. nov. is proposed. The type strain is MANO22D T (=KCTC 12520 T =DSM 17657 T ). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains MANO22D T and MANO22P are DQ097523 and DQ097524 , respectively.