A simple and effective coumarin-based fluorescent probe for cysteine

• Published in: Biosensors & bioelectronics Vol. 59; pp. 35 - 39
• Main Authors: Dai, Xi, Wu, Qing-Hua, Wang, Peng-Chong, Tian, Jie, Xu, Yu, Wang, Sheng-Qing, Miao, Jun-Ying, Zhao, Bao-Xiang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.09.2014; Elsevier
• Subjects: Acrylic acid; Animals; Biological and medical sciences; Biosensing Techniques - methods; Biotechnology; Calf serum; Cattle; Cell imaging; Conjugate addition; Conjugates; Coumarin; Coumarins - chemistry; Cysteine; Cysteine - analysis; Cysteine - blood; Esters; Fluorescence; Fluorescent Dyes - chemistry; Fluorescent probe; Fundamental and applied biological sciences. Psychology; HeLa Cells; Humans; Limit of Detection; Optical Imaging - methods; Spectra; Spectrometry; Spectrometry, Fluorescence - methods; Calf serum; Conjugate addition; Cysteine; Cell imaging; Coumarin; Fluorescent probe; Calf; Fluorescence; Ox; Imagery; Probe; Vertebrata; Mammalia; Serum; Artiodactyla; Ungulata
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2014.03.018

Acrylic acid 3-acetyl-2-oxo-2 H-chromen-7-yl ester (ACA) was rationally designed and synthesized as a simple and effective fluorescent probe for sensing cysteine with high selectivity and naked-eye detection. The probe can detect cysteine by fluorescence spectrometry with a detection limit of 0.657μM and can be used with calf serum and in live cell imaging. The conjugate addition/cyclization sequence mechanism of the reaction between ACA and cysteine was confirmed by ESI-MS and fluorescence spectra. •A simple and effective coumarin-based fluorescent probe for cysteine was synthesized.•The probe can detect cysteine with high selectivity and sensitivity in aqueous solution.•The probe was successfully used for detecting Cys in calf serum and living imaging.