Genetic variants near TIMP3 and high-density lipoprotein-associated loci influence susceptibility to age-related macular degeneration
We executed a genome-wide association scan for age-related macular degeneration (AMD) in 2,157 cases and 1,150 controls. Our results validate AMD susceptibility loci near CFH (P < 10⁻⁷⁵), ARMS2 (P < 10⁻⁵⁹), C2/CFB (P < 10⁻²⁰), C3 (P < 10⁻⁹), and CFI (P < 10⁻⁶). We compared our top fin...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 107; no. 16; pp. 7401 - 7406 |
---|---|
Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
20.04.2010
National Acad Sciences |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | We executed a genome-wide association scan for age-related macular degeneration (AMD) in 2,157 cases and 1,150 controls. Our results validate AMD susceptibility loci near CFH (P < 10⁻⁷⁵), ARMS2 (P < 10⁻⁵⁹), C2/CFB (P < 10⁻²⁰), C3 (P < 10⁻⁹), and CFI (P < 10⁻⁶). We compared our top findings with the Tufts/Massachusetts General Hospital genome-wide association study of advanced AMD (821 cases, 1,709 controls) and genotyped 30 promising markers in additional individuals (up to 7,749 cases and 4,625 controls). With these data, we identified a susceptibility locus near TIMP3 (overall P = 1.1 x 10⁻¹¹), a metalloproteinase involved in degradation of the extracellular matrix and previously implicated in early-onset maculopathy. In addition, our data revealed strong association signals with alleles at two loci (LIPC, P = 1.3 x 10⁻⁷; CETP, P = 7.4 x 10⁻⁷) that were previously associated with high-density lipoprotein cholesterol (HDL-c) levels in blood. Consistent with the hypothesis that HDL metabolism is associated with AMD pathogenesis, we also observed association with AMD of HDL-c--associated alleles near LPL (P = 3.0 x 10⁻³) and ABCA1 (P = 5.6 x 10⁻⁴). Multilocus analysis including all susceptibility loci showed that 329 of 331 individuals (99%) with the highest-risk genotypes were cases, and 85% of these had advanced AMD. Our studies extend the catalog of AMD associated loci, help identify individuals at high risk of disease, and provide clues about underlying cellular pathways that should eventually lead to new therapies. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: W.C., D.S., A.O.E., K.E.B., M.O., J.J., N.T., P.L.T., Y.P.C., A.K., L.K., Y.L., K.J.A., A.J.K., W.K.S., A.A., J.L.K., S.G.S., E.A.P., M. Brooks, K.H.B., W.L.B., A.J.B., A.O., G.B., A.H., C.R., L.D., L.T., B.K., D.H., B.E.K.K., F.F., J.A.F., E.W.P., K.F.D., M.M.D., D.E.W., D.J.Z., E.Y.C., J.R.H., S.K.I., P.J.F., N.K., J.M.S., J.L.H., M.B.G., G.R.A., and A.S. performed research; D.S., A.O.E., K.E.B., M.O., G.R.A., and A.S. designed research; D.S., M.A.P.-V., P.A.C., M.L.K., A.K., L.K., CAPT Research Group, S.A.H., N.S.P., R.K., N.G., K.Y., J.A.F., R.R., L.A.F., I.K.K., J.W.M., M.C., A.C., M.S.-S., M. Brion, M.M.D., D.E.W., D.J.Z., N.Y., S.K.I., P.J.F., N.K., J.M.S., J.L.H., M.B.G., and A.S. contributed new reagents/analytic tools; W.C., J.J., N.T., L.K., Y.L., M. Brion, S.K.I., G.R.A., and A.S. analyzed data; and W.C., D.S., A.O.E., G.R.A., and A.S. wrote the paper. 1W.C., D.S, and A.O.E. contributed equally to this work. Edited by Mark T. Keating, Novartis Institute for Biomedical Research, Cambridge, MA, and approved March 4, 2010 (received for review November 11, 2009) |
ISSN: | 0027-8424 1091-6490 1091-6490 |
DOI: | 10.1073/pnas.0912702107 |