The expression of antioxidant enzymes in the gingivae of type 2 diabetics with chronic periodontitis

• Published in: Archives of oral biology Vol. 57; no. 2; pp. 161 - 168
• Main Authors: Duarte, Poliana M., Napimoga, Marcelo H., Fagnani, Ellen C., Santos, Vanessa R., Bastos, Marta F., Ribeiro, Fernanda V., Araújo, Vera C., Demasi, Ana Paula D.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2012
• Subjects: Adult; Advanced Basic Science; Antioxidant; Antioxidants - metabolism; Biopsy; Case-Control Studies; Catalase - genetics; Catalase - metabolism; Chronic Periodontitis - complications; Chronic Periodontitis - enzymology; Dentistry; Diabetes mellitus; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - enzymology; Female; Gene Expression; Gingiva - enzymology; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Humans; Inflammation; Male; Middle Aged; Oxidative Stress; Periodontitis; Peroxiredoxins - genetics; Peroxiredoxins - metabolism; Polymerase Chain Reaction; RNA, Messenger - metabolism; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Oxidative stress; Inflammation; Periodontitis; Antioxidant; Diabetes mellitus
• ISSN: 0003-9969; 1879-1506; 1879-1506
• DOI: 10.1016/j.archoralbio.2011.08.007

There is controversial evidence regarding the levels of antioxidant molecules in type 2 diabetes periodontitis patients. Thus, the aim of the present study was to evaluate the gene expression of antioxidant enzymes in the gingival tissue of poorly and well-controlled type 2 diabetic subjects with chronic periodontitis (CP). Gingival biopsies were harvested from systemically and periodontally healthy subjects (n=12), systemically healthy subjects with CP (n=15), well-controlled (n=8) and poorly controlled (n=14) diabetic subjects with CP. The messenger RNA (mRNA) levels of peroxiredoxin (PRDX) 1 and 2, catalase (CAT), glutathione peroxidase (GPX1) and superoxide dismutase (SOD) 1 and 2 were measured by quantitative polymerase chain reaction (qPCR). The results showed that PRDX1 and GPX1 were up-regulated by periodontitis (p<0.05), independently of the glycaemic status, whilst PRDX2 and SOD2 genes were slightly influenced by periodontitis, but significantly induced when periodontitis was associated with DM, especially under a poor glycaemic control (p<0.05). Moreover, CAT and SOD1 expressions were not significantly influenced by any of these inflammatory disorders (p>0.05). In conclusion, both PRDX1 and GPX1 were overexpressed in CP whilst PRDX2 and SOD2 were up-regulated especially in the poorly controlled diabetic group with CP.