Characterization of stress indicators in Tilia cordata Mill. as early and long-term stress markers for water availability and trace element contamination in urban environments

• Published in: Ecological indicators Vol. 158; p. 111296
• Main Authors: Andrianjara, Iry, Cabassa, Cécile, Lata, Jean-Christophe, Hansart, Amandine, Raynaud, Xavier, Renard, Mathilde, Nold, Francois, Genet, Patricia, Planchais, Séverine
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.01.2024; Elsevier
• Subjects: climate change; Controlled condition experiment; Environmental Sciences; France; genus; lead; Malondialdehyde; photosynthesis; Photosynthetic pigments; Proline; rehydration; soil; soil compaction; soil pollution; Tilia cordata; trace elements; trees; water stress; France; Proline; Controlled condition experiment; Photosynthetic pigments; Malondialdehyde
• ISSN: 1470-160X; 1872-7034
• DOI: 10.1016/j.ecolind.2023.111296

[Display omitted] •Collaboration between scientists and urban managers must be encouraged to monitor tree health.•Effective early indicators are needed to check urban tree’s phytosanitary status.•Some plant chemical compounds have proven useful to detect abiotic stresses in Tilia genus.•Chlorophyll content decrease was a marker of some trace element contamination and water stress.•Proline and malondialdehyde increases were markers for water stress. In the context of current climate change, municipalities tend to plant more trees in order to benefit from services they provide in urban areas, such as reducing heat islands. However, in cities, trees are subject to severe abiotic constraints, the main ones being soil contamination and the lack of water due to soil compaction and droughts. This can negatively impact the ecological services provided by trees. Our study aims to help managers in monitoring the phytosanitary status of trees in order to be able to react promptly. In a controlled condition experiment, different plant compounds were analyzed (proline, malondialdehyde-MDA, photosynthetic pigments) in the leaves of young linden trees conventionally grown for planting by the City of Paris, France. They were grown on a trace element (TE) contamination soil gradient, and under different water regimes (well-hydrated/no water stress (Ct), dehydration (Dh), rehydration (Rh)) to see if tested compounds could be early stress markers in trees. Chlorophylls (Chl) could be considered as water and TE-mediated stress markers. Indeed Chl a and b concentrations significantly decreased with dehydration (e.g. respectively −34 % and −24 %) and increasing lead concentration in leaves (respectively, correlation coefficients were −1.08; −0.64 (p < 0.001)). On the contrary, Chl concentrations increased with increasing copper concentration in optimal physiological ranges (correlation coefficients for Chla and Chb were respectively 1.3 and 1.08 (p < 0.05)). Proline and malondialdehyde seemed to be good complementary markers of water stress in the Tilia genus. Indeed, proline concentration increased during the dehydration period (early water stress marker) (mean concentration for Ct, Dh and Rh trees were respectively 3.6, 29.6 and 51.3 µmol.g−1 DW), while MDA increased during the rehydration process (marker for stress accumulation over time) (mean concentration for Ct, Dh, and Rh were respectively 461.8, 313.9 and 493.5 nmol.g−1 DW). In order to reinforce the diagnoses of urban tree managers, these stress indicators should be tested in situ.