CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines

• Published in: Molecular human reproduction Vol. 14; no. 7; pp. 423 - 430
• Main Authors: Dominguez, F., Martínez, S., Quiñonero, A., Loro, F., Horcajadas, J.A., Pellicer, A., Simón, C.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.07.2008; Oxford Publishing Limited (England)
• Subjects: Adult; Biological and medical sciences; Blastocyst - cytology; Blastocyst - drug effects; Blastocyst - metabolism; Cell Line; Cell Movement - drug effects; Chemokine CXCL10 - genetics; Chemokine CXCL10 - metabolism; Chemokine CXCL10 - pharmacology; chemotaxis; Chemotaxis - drug effects; Culture Media, Conditioned - pharmacology; CXCL10; Embryology: invertebrates and vertebrates. Teratology; Endometrium - cytology; Endometrium - drug effects; Endometrium - metabolism; Female; Fundamental and applied biological sciences. Psychology; Humans; IL-6; Immunohistochemistry; implantation; Interleukin-6 - pharmacology; Menstrual Cycle - metabolism; Protein Array Analysis; Receptors, CXCR3 - genetics; Receptors, CXCR3 - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Ruminantia; Trophoblasts - cytology; Trophoblasts - drug effects; Trophoblasts - metabolism; CXCL10; implantation; IL-6; chemotaxis; Pregnancy; Human; Interleukin 6; Trophoblaste; Cell line; Ovum implantation; Fetal membrane; Cytokine; Chemotaxis
• ISSN: 1360-9947; 1460-2407
• DOI: 10.1093/molehr/gan032

The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-γ-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophoblast cells during early gestation. The aim of this work was to explore the existence of CXCL10/CXCR3 in the human model. Furthermore, chemotaxis assays were performed to demonstrate CXCL10 chemotactic activity in the human trophoblast cell lines JEG-3 and AC-1M88. Surprisingly, the conditioned media from epithelial endometrial cells (EEC) induced the highest trophoblast migration rate. Cytokine and chemokine membrane protein arrays were used to identify the secreted protein profile of EEC-conditioned media, and IL-6 was found to be the most abundant and CXCL13 the second most abundant molecule. Using a chemotaxis assay on AC-IM88, IL-6 antibody blocked the effect of EEC, indicating IL-6 to be an effective chemoattractive factor for trophoblast cells in the human model.