CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines
The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-γ-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophob...
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Published in | Molecular human reproduction Vol. 14; no. 7; pp. 423 - 430 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
01.07.2008
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-γ-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophoblast cells during early gestation. The aim of this work was to explore the existence of CXCL10/CXCR3 in the human model. Furthermore, chemotaxis assays were performed to demonstrate CXCL10 chemotactic activity in the human trophoblast cell lines JEG-3 and AC-1M88. Surprisingly, the conditioned media from epithelial endometrial cells (EEC) induced the highest trophoblast migration rate. Cytokine and chemokine membrane protein arrays were used to identify the secreted protein profile of EEC-conditioned media, and IL-6 was found to be the most abundant and CXCL13 the second most abundant molecule. Using a chemotaxis assay on AC-IM88, IL-6 antibody blocked the effect of EEC, indicating IL-6 to be an effective chemoattractive factor for trophoblast cells in the human model. |
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Bibliography: | istex:82902A2D4C7969440C6C534F89BDB9BF70898B82 ArticleID:gan032 ark:/67375/HXZ-SMXRLQGR-B ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 1360-9947 1460-2407 |
DOI: | 10.1093/molehr/gan032 |