Analysis of outer membrane vesicle protein involved in biofilm formation of Helicobacter pylori

• Published in: Anaerobe Vol. 17; no. 6; pp. 388 - 390
• Main Authors: Yonezawa, Hideo, Osaki, Takako, Woo, Timothy, Kurata, Satoshi, Zaman, Cynthia, Hojo, Fuhito, Hanawa, Tomoko, Kato, Shuichi, Kamiya, Shigeru
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2011
• Subjects: Bacterial Adhesion; bacterial infections; Bacterial Outer Membrane Proteins - analysis; biofilm; Biofilm formation; Biofilms - growth & development; gastritis; glass; Helicobacter pylori; Helicobacter pylori - growth & development; Helicobacter pylori - metabolism; Helicobacter pylori - physiology; human diseases; lymphoma; OMV; peptic ulcers; Proteome - analysis; risk factors; Secretory Vesicles - chemistry; stomach neoplasms; Helicobacter pylori; Biofilm formation; OMV
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2011.03.020

Helicobacter pylori is one of the most common causes of bacterial infection in humans. Infection with H. pylori is closely associated with gastritis and peptic ulcers and is a risk factor for gastric cancer and mucosa-associated lymphoid tissue lymphoma. H. pylori forms biofilms on glass surfaces at the air–liquid interface in in-vitro batch cultures. We previously reported that strain TK1402 showed a strong biofilm-forming ability in vitro. We also suggested the outer membrane vesicles (OMV) produced by strain TK1402 might be related to its biofilm forming ability. In the present study, we analyzed the protein profile of the OMV produced by strain TK1402 and found a unique 22-kDa protein in TK1402 OMV cultured for 2–3 days. In addition, this protein could not be detected in the OMVs produced by other H. pylori strains. These results suggest that the 22-kDa protein is involved in effective biofilm formation by strain TK1402.