In vivo detection of lamellocytes in Drosophila melanogaster

Abstract Drosophila has recently become a powerful model organism for studies of innate immunity. The cellular elements of innate immunity in Drosophila , the hemocytes, have been characterized by morphological criteria, molecular markers, and cell-type-specific immunological markers. Here we sugges...

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Bibliographic Details
Published inImmunology letters Vol. 126; no. 1; pp. 83 - 84
Main Authors Honti, Viktor, Kurucz, Éva, Csordás, Gábor, Laurinyecz, Barbara, Márkus, Róbert, Andó, István
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 22.09.2009
Subjects
Allergy and Immunology
Animals
Cell Differentiation
Differentiation
DNA Transposable Elements - genetics
Drosophila
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Fluorescent Antibody Technique, Indirect
Genetic screening
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hemocyte
Hemocyte marker
Hemocytes
Hemocytes - cytology
Hemocytes - metabolism
Immunity
In vivo
Insertion
Lamellocyte
Larva - genetics
Larva - metabolism
Mutagenesis, Insertional
In vivo
Hemocyte
Lamellocyte
Differentiation
Drosophila
Hemocyte marker
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Summary:Abstract Drosophila has recently become a powerful model organism for studies of innate immunity. The cellular elements of innate immunity in Drosophila , the hemocytes, have been characterized by morphological criteria, molecular markers, and cell-type-specific immunological markers. Here we suggest that an MiET1 GFP-reporter element insertion in the untranslated region of a gene ( l1 - atilla ) – expressed in a subset of hemocytes, the lamellocytes – allows in vivo investigations of lamellocyte differentiation and facilitates genetic screens.
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ISSN:0165-2478
1879-0542
DOI:10.1016/j.imlet.2009.08.004