Partitioning of Thy-1, GM1, and Cross-Linked Phospholipid Analogs into Lipid Rafts Reconstituted in Supported Model Membrane Monolayers
As shown earlier, raft-like domains resembling those thought to be present in natural cell membranes can be formed in supported planar lipid monolayers. These liquid-ordered domains coexist with a liquid-disordered phase and form in monolayers prepared both from synthetic lipid mixtures and lipid ex...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 98; no. 19; pp. 10642 - 10647 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
11.09.2001
National Acad Sciences The National Academy of Sciences |
Series | From the Cover |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | As shown earlier, raft-like domains resembling those thought to be present in natural cell membranes can be formed in supported planar lipid monolayers. These liquid-ordered domains coexist with a liquid-disordered phase and form in monolayers prepared both from synthetic lipid mixtures and lipid extracts of the brush border membrane of mouse kidney cells. The domains are detergent-resistant and are highly enriched in the glycosphingolipid GM1. In this work, the properties of these raft-like domains are further explored and compared with properties thought to be central to raft function in plasma membranes. First, it is shown that domain formation and disruption critically depends on the cholesterol density and can be controlled reversibly by treating the monolayers with the cholesterol-sequestering reagent methyl-β-cyclodextrin. Second, the glycosylphosphatidylinositol-anchored cell-surface protein Thy-1 significantly partitions into the raft-like domains. The extent of this partitioning is reduced when the monolayers contain GM1, indicating that different molecules can compete for domain occupation. Third, the partitioning of a saturated phospholipid analog into the raft phase is dramatically increased (15% to 65%) after cross-linking with antibodies, whereas the distribution of a doubly unsaturated phospholipid analog is not significantly affected by cross-linking (≈10%). This result demonstrates that cross-linking, a process known to be important for certain cell-signaling processes, can selectively translocate molecules to liquid-ordered domains. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by Kai Simons, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, and approved July 2, 2001 To whom reprint requests should be addressed at: Department of Cell and Developmental Biology, University of North Carolina, CB# 7090, 108 Taylor Hall, Chapel Hill, NC 27599. E-mail: frap@med.unc.edu. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.191168698 |