Metabolite profile deviations in an oral glucose tolerance test—a comparison between lean and obese individuals
Objective While impaired glucose tolerance diagnosed by the oral glucose tolerance test (OGTT) is a common trait in obese individuals, less is known about changes in levels of other metabolites. The aim was to reveal the complex alterations in metabolite levels provoked by an OGTT and its perturbati...
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Published in | Obesity (Silver Spring, Md.) Vol. 22; no. 11; pp. 2388 - 2395 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.11.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Objective
While impaired glucose tolerance diagnosed by the oral glucose tolerance test (OGTT) is a common trait in obese individuals, less is known about changes in levels of other metabolites. The aim was to reveal the complex alterations in metabolite levels provoked by an OGTT and its perturbation in obese individuals.
Methods
Gas chromatography/mass spectrometry was used to profile metabolite levels in serum from 14 obese participants (body mass index [BMI] of 43.6 ± 1.5 kg m−2 [mean ± SEM]) at 0, 30, and 120 min during a standard 2‐h 75 g OGTT. Metabolite profiles from six lean individuals (BMI of 22.4 ± 2.4 kg m−2), collected from a previous study, were included for comparison.
Results
In the obese group, 59 metabolite profiles were determined. Among these, 16 deviated from profiles in the lean group. Deviating metabolites were categorized into three groups. Delayed reduction in levels of five fatty acids. Increased levels at 30 min of five amino acids, including isoleucine and leucine. A blunted increase at 30 min of six metabolites.
Conclusions
Metabolomics analysis revealed distinct differences in alterations of metabolite levels during an OGTT in obese and lean subjects. To this end, our data suggests a disrupted regulation of ketogenesis, lipolysis and proteolysis in obese individuals. |
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Bibliography: | MR and AD designed the study. MR collected the data from participants. NG and AD carried out the experiments and analyzed the data. NG generated tables and figures. PS, AD, HM and KF performed the study on the lean group, analyzed the data and co‐wrote the manuscript. NG, MR, AD wrote the manuscript. All authors approved the final version. The authors have no competing interests. Disclosures This study was funded by the Swedish Research Council, Novo Nordisk Foundation, the Crafoord Foundation, Skåne University Hospital Foundation, the Albert Påhlsson Foundation, the Lundberg Foundation, the Diabetes Association in Malmö, Region Skåne, ALF, the Magnus Bergvall Foundation, the Fredrik and Ingrid Thurings Foundation, the Borgströms Foundation, the Lars Hierta Foundation, and the Diabetes program at Lund University. Funding agencies Author contributions ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1930-7381 1930-739X 1930-739X |
DOI: | 10.1002/oby.20868 |