Fractionation of Aspergillus niger cellulases by combined ion exchange affinity chromatography
Eight chemically modified cellulose supports were tested for their ability to absorb components of the Aspergillus niger cellulase system. At least two of the most effective adsorbents, aminoethyl cellulose and carboxymethyl cellulose, were shown to be useful for the fractionation of cellulases. The...
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Published in | Biotechnology and bioengineering Vol. 29; no. 2; p. 176 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
05.02.1987
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Subjects | |
Online Access | Get more information |
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Summary: | Eight chemically modified cellulose supports were tested for their ability to absorb components of the Aspergillus niger cellulase system. At least two of the most effective adsorbents, aminoethyl cellulose and carboxymethyl cellulose, were shown to be useful for the fractionation of cellulases. These supports apparently owe their resolving capacity to both ion exchange and biospecific binding effects; however, the relative importance of each effect is unknown. These observations form the basis for a new cellulase fractionation technique, combined ion exchange-affinity chromatography. |
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Bibliography: | F60 8726582 |
ISSN: | 0006-3592 1097-0290 |
DOI: | 10.1002/bit.260290206 |