Fractionation of Aspergillus niger cellulases by combined ion exchange affinity chromatography

Eight chemically modified cellulose supports were tested for their ability to absorb components of the Aspergillus niger cellulase system. At least two of the most effective adsorbents, aminoethyl cellulose and carboxymethyl cellulose, were shown to be useful for the fractionation of cellulases. The...

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Published inBiotechnology and bioengineering Vol. 29; no. 2; p. 176
Main Authors Boyer, R.F, Allen, T.L, Dykema, P.A
Format Journal Article
LanguageEnglish
Published United States 05.02.1987
Subjects
ASPERGILLUS
CELLULASE
CELULASA
CHROMATOGRAPHIE
CHROMATOGRAPHY
CROMATOGRAFIA
ECHANGE D'ION
INTERCAMBIO IONICO
ION EXCHANGE
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Summary:Eight chemically modified cellulose supports were tested for their ability to absorb components of the Aspergillus niger cellulase system. At least two of the most effective adsorbents, aminoethyl cellulose and carboxymethyl cellulose, were shown to be useful for the fractionation of cellulases. These supports apparently owe their resolving capacity to both ion exchange and biospecific binding effects; however, the relative importance of each effect is unknown. These observations form the basis for a new cellulase fractionation technique, combined ion exchange-affinity chromatography.
Bibliography:F60
8726582
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260290206