Strain selection and medium optimization for glucoamylase production from industrial potato waste by Aspergillus niger

BACKGROUND Glucoamylase is one of the most common enzymes used in the food industry to break down starch into its monomers. Glucoamylase production and its activity are highly dependent on medium composition. Starch is well known as a glucoamylase inducer, and utilization of industrial starchy potat...

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Published inJournal of the science of food and agriculture Vol. 96; no. 8; pp. 2788 - 2795
Main Authors Izmirlioglu, Gulten, Demirci, Ali
Format Journal Article
LanguageEnglish
Published Chichester, UK John Wiley & Sons, Ltd 01.06.2016
John Wiley and Sons, Limited
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Summary:BACKGROUND Glucoamylase is one of the most common enzymes used in the food industry to break down starch into its monomers. Glucoamylase production and its activity are highly dependent on medium composition. Starch is well known as a glucoamylase inducer, and utilization of industrial starchy potato waste is an inexpensive way of improving glucoamylase production. Since glucoamylase production is highly dependent on medium composition, in this study medium optimization for glucoamylase production was considered to enhance glucoamylase activity. RESULTS Among the evaluated microbial species, Aspergillus niger van Tieghem was found to be the best glucoamylase‐producing fungus. The Plackett–Burman design was used to screen various medium ingredients, and malt extract, FeSO4.7H2O and CaCl2·2H2O were found to have significant effects on glucoamylase production. Finally, malt extract, FeSO4.7H2O and CaCl2.2H2O were optimized by using a central composite design of response surface methodology. The results showed that the optimal medium composition for A. niger van Tieghem was 50 g L−1 industrial waste potato mash supplemented with 51.82 g L−1 malt extract, 9.27 g L−1 CaCl2·2H2O and 0.50 g L−1 FeSO4.7H2O. CONCLUSION At the end of optimization, glucoamylase activity and glucose production were improved 126% and 98% compared to only industrial waste potato mash basal medium; 274.4 U mL−1 glucoamylase activity and 41.7 g L−1 glucose levels were achieved, respectively. © 2015 Society of Chemical Industry
Bibliography:ark:/67375/WNG-Z5VRGS48-5
istex:02F0730B79D50620EABE277ABEE628EA19B9F0FC
Pennsylvania Agricultural Experiment Station
Presented as a poster at the Institute of Food Technologists (IFT) 2014 annual meeting.
Turkish Ministry of Education
ArticleID:JSFA7445
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-5142
1097-0010
DOI:10.1002/jsfa.7445