Gene Mapping with Recombinant Inbreds in Maize

• Published in: Genetics (Austin) Vol. 118; no. 3; pp. 519 - 526
• Main Authors: Burr, B, Burr, F. A, Thompson, K. H, Albertson, M. C, Stuber, C. W
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Genetics Soc America 01.03.1988; Genetics Society of America
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Chromosome Mapping; Classical and quantitative genetics. Population genetics. Molecular genetics; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genes; Genetics and breeding of economic plants; Genetics of eukaryotes. Biological and molecular evolution; Inbreeding; Investigations; Plants - genetics; Pteridophyta, spermatophyta; Recombination, Genetic; Vegetals; Zea mays; Zea mays - genetics; Genetic mapping; Monocotyledones; Zea mays; Mitotic recombination; Gramineae; Angiospermae; F2 hybrid; Inbreeding; Spermatophyta; Genome; Cereal crop; Polymorphism
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/118.3.519

Recombinant inbred lines of maize have been developed for the rapid mapping of molecular probes to chromosomal location. Two recombinant inbred families have been constructed from F2 populations of T232 X CM37 and CO159 X Tx303. A genetic map based largely on isozymes and restriction fragment length polymorphisms has been produced that covers virtually the entire maize genome. In order to map a new gene, an investigator has only to determine its allelic distribution among the recombinant inbred lines and then compare it by computer with the distributions of all previously mapped loci. The availability of the recombinant inbreds and the associated data base constitute an efficient means of mapping new molecular markers in maize.