Impact of fermentation conditions on the production of bioactive compounds with anticancer, anti-inflammatory and antioxidant properties in kombucha tea extracts

• Published in: Process biochemistry (1991) Vol. 83; pp. 44 - 54
• Main Authors: Villarreal-Soto, Silvia Alejandra, Beaufort, Sandra, Bouajila, Jalloul, Souchard, Jean-Pierre, Renard, Thierry, Rollan, Serge, Taillandier, Patricia
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01.08.2019; Elsevier BV; Elsevier
• Subjects: Acetic acid; anti-inflammatory activity; Anti-inflammatory agents; Anticancer properties; antineoplastic activity; antioxidant activity; Antioxidants; Antitumor activity; Bioactive compounds; Biological activity; Biotechnology; Black tea; Cancer; Chemical and Process Engineering; Chemical engineering; Chemical Sciences; Colon; colorectal neoplasms; Consortia; Engineering Sciences; Ethyl acetate; Fermentation; gas chromatography-mass spectrometry; geometry; human cell lines; humans; Inflammation; inhibitory concentration 50; Kombucha; Life Sciences; Medicinal Chemistry; neoplasm cells; Organic acids; organic acids and salts; phenolic compounds; Phenols; Plants (botany); Polarity; Solvents; Tea; CAFF; C; MCF-7; NSAID; TPC; Kombucha; HCT-116; TAX; MTT; Black tea; s/h; TMCS; Bioactive compounds; BSTFA; GA; s/v; CGA; CA; EC; HPLC; LOD; R2; CAA; DPPH; EGCG; ECG; Fermentation; TB; LOQ; RUT; TCA; LOX; NDGA; UPLC; RI; FA; BHB
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2019.05.004

[Display omitted] •Improvement of the biological activities after fermentation with the consortium.•A better antiproliferation effect was obtained with a higher fermentation ratio s/h.•Forty-seven organic compounds were identified by GC–MS.•Identification of a key variable in the fermentation of kombucha tea. The production of natural bioactive compounds through the fermentation of plants has increased in recent years. The biological activities of the extracts obtained from the fermentation of black tea with the kombucha consortium were evaluated. To improve the productivity of these compounds two different vessel geometries were used and successive extractions with solvents of increasing polarity were performed. Forty-seven compounds were identified by GC–MS, including several organic acids and phenolic compounds. Total phenolic content, pH value, and antioxidant, antiproliferation and anti-inflammatory activities were measured after 21 days of fermentation. A higher surface/height (s/h) ratio seemed to enhance the anti-inflammatory activity of kombucha tea, resulting in IC50 value of 9.0 ± 0.1 μg/mL compared to 24.3 ± 0.2 μg/mL with the lowest ratio. Regarding the anticancer activity, the highest inhibition percentage of 55.3% at 50 μg/mL against the HCT-116 human colon carcinoma cell line was obtained with the ethyl acetate extract after 21 days of fermentation compared to the value of 8% obtained with the same extraction solvent using the non-fermented black tea. These results showed that fermentation may improve the biological activities of the tea and that the production of bioactive compounds can vary depending on the fermentation conditions.