Plasma treatment of paper for protein immobilization on paper-based chemiluminescence immunodevice

• Published in: Biosensors & bioelectronics Vol. 79; pp. 581 - 588
• Main Authors: Zhao, Mei, Li, Huifang, Liu, Wei, Guo, Yumei, Chu, Weiru
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.05.2016
• Subjects: Aldehydes; Antibodies; Antibodies, Immobilized - chemistry; Biosensing Techniques - instrumentation; Carcinoembryonic Antigen - blood; Chemiluminescence; Equipment Design; Fourier transforms; Humans; Immobilization; Immunoassay - instrumentation; Limit of Detection; Luminescence; Luminescent Measurements - instrumentation; Oxygen - chemistry; Oxygen plasma treatment; paper-based immunodevice; Plasma Gases - chemistry; protein immobilization; Proteins; Spectra; protein immobilization; paper-based immunodevice; chemiluminescence; Oxygen plasma treatment
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.12.099

A novel protein immobilization method based on plasma treatment of paper on the low-cost paper-based immunodevice was established in this work. By using a benchtop plasma cleaner, the paper microzone was treated by oxygen plasma treatment for 4min and then the antibody can be directly immobilized on the paper surface. Aldehyde group was produced after the plasma treatment, which can be verified from the fourier transform infrared spectroscopy (FT-IR) spectra and x-ray photoelectron spectroscopy (XPS) spectra. By linked to aldehyde group, the antibody can be immobilized on the paper surface without any other pretreatment. A paper-based immunodevice was introduced here through this antibody immobilization method. With sandwich chemiluminescence (CL) immunoassay method, the paper-based immunodevice was successfully performed for carcinoembryonic antigen (CEA) detection in human serum with a linear range of 0.1–80.0ng/mL. The detection limit was 0.03ng/mL, which was 30 times lower than the clinical CEA level. Comparing to the other protein immobilization methods on paper-based device, this strategy was faster and simpler and had potential applications in point-of-care testing, public health and environmental monitoring. •A protein immobilization method based on plasma treatment of paper was developed.•Aldehyde group was produced on the paper after the plasma treatment.•The plasma treated time was 4min and the protein immobilization strategy was faster.•Carcinoembryonic antigen was detected by this paper-based immunodevice.