A comparative protein profiling of lymphocytes from blood of patients with chronic lymphoid leukemia by high-resolution mass-spectrometry in search of new markers for heterogeneity and disease prognosis

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 71; no. 13; pp. 1242 - 1250
• Main Authors: Glazyrin, Yu. E., Komarova, M. A., Bakhtina, V. I., Silacheva, M. V., Demko, I. V., Zamay, A. S., Zamay, T. N.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 15.12.2016; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Care and treatment; Chemistry; Chemistry and Materials Science; Comparative analysis; Diagnosis; Heterogeneity; Heterogeneous catalysis; High resolution; Leukemia; Lymphocytes; Markers; Mass spectrometry; Prognosis; Proteins; Quantitative analysis; Scientific imaging; Spectroscopy; Statistical analysis; lymphocytes; proteomics; profiling; chronic lymphoid leukemia; proteins; biomarkers; mass spectrometry
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934816130062

To find new markers of chronic lymphoid leukemia heterogeneity, a comparison of the proteomic profiles of pathologic and healthy lymphocytes from blood was carried out by high-resolution mass spectrometry and label-free quantitative analysis. A list of the most typical proteins for the two groups was obtained. The differences in the proteomic profiles within the pathologic group, which were consistent with the heterogeneity of clinical background, were found. Two subsets within the area of the pathologic cells were formed using statistical analysis; the list of characteristic proteins for each subset was obtained.