Nanozyme-strip for rapid local diagnosis of Ebola

• Published in: Biosensors & bioelectronics Vol. 74; pp. 134 - 141
• Main Authors: Duan, Demin, Fan, Kelong, Zhang, Dexi, Tan, Shuguang, Liang, Mifang, Liu, Yang, Zhang, Jianlin, Zhang, Panhe, Liu, Wei, Qiu, Xiangguo, Kobinger, Gary P., Fu Gao, George, Yan, Xiyun
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.12.2015
• Subjects: Biotechnology; Bunyavirus; Diagnosis; Ebola detection; Ebola virus; Ebolavirus - isolation & purification; ELISA; Equipment Design; Glycoproteins - isolation & purification; Hemorrhagic Fever, Ebola - diagnosis; Hemorrhagic Fever, Ebola - virology; Humans; Immunochromatographic strip; Immunochromatography - instrumentation; Magnetite Nanoparticles - chemistry; Nanostructure; Nanozyme; Reagent Strips - analysis; Sensitivity and Specificity; Strip; Vaccines; Viral Proteins - isolation & purification; Immunochromatographic strip; Nanozyme; Ebola detection
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.05.025

Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas. •We developed a Nanozyme-strip method, which is 100 times more sensitive than the colloidal gold strip.•The first report of introducing Fe3O4 nanozyme into immunochromatographic strip.•The novel method could be used as a valuable tool for diagnosis of Ebola and other infectious disease.