Both adiponectin and interleukin-10 inhibit LPS-induced activation of the NF-κB pathway in 3T3-L1 adipocytes

• Published in: Cytokine (Philadelphia, Pa.) Vol. 57; no. 1; pp. 98 - 106
• Main Authors: Lira, Fábio Santos, Rosa, José Cesar, Pimentel, Gustavo Duarte, Seelaender, Marília, Damaso, Ana Raimunda, Oyama, Lila Missae, Oller do Nascimento, Claudia
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2012
• Subjects: 3T3-L1; 3T3-L1 Cells; adipocytes; Adipocytes - drug effects; Adipocytes - metabolism; Adiponectin; Adiponectin - pharmacology; Animals; Culture Media; Culture Media - pharmacology; DNA; DNA - metabolism; drug effects; energy; homeostasis; inflammation; insulin resistance; Interleukin 10; Interleukin-10 - pharmacology; interleukin-6; Interleukin-6 - secretion; Lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - pharmacology; metabolism; Mice; Myeloid Differentiation Factor 88; Myeloid Differentiation Factor 88 - metabolism; NF-kappa B; NF-kappa B - metabolism; NF-κB pathway; pharmacology; Protein Binding; Protein Binding - drug effects; protein synthesis; proteins; secretion; signal transduction; Signal Transduction - drug effects; Time Factors; TNF Receptor-Associated Factor 6; TNF Receptor-Associated Factor 6 - metabolism; transcription factor NF-kappa B; Lipopolysaccharide; 3T3-L1; Interleukin 10; NF-κB pathway; Adiponectin
• ISSN: 1043-4666; 1096-0023; 1096-0023
• DOI: 10.1016/j.cyto.2011.10.001

► The effects of adiponectin and IL-10 on NF-κB DNA binding activity. ► Role of TLR signaling in LPS-treated 3T3-L1 adipocytes. ► Role the adiponectin and IL-10 upon TLR signaling. ► Action alone and/or combination the adiponectin and IL-10. Adiponectin and interleukin 10 (IL-10) are adipokines that are predominantly secreted by differentiated adipocytes and are involved in energy homeostasis, insulin sensitivity, and the anti-inflammatory response. These two adipokines are reduced in obese subjects, which favors increased activation of nuclear factor kappa B (NF-κB) and leads to elevation of pro-inflammatory adipokines. However, the effects of adiponectin and IL-10 on NF-κB DNA binding activity (NF-κBp50 and NF-κBp65) and proteins involved with the toll-like receptor (TLR-2 and TLR-4) pathway, such as MYD88 and TRAF6 expression, in lipopolysaccharide-treated 3T3-L1 adipocytes are unknown. Stimulation of lipopolysaccharide-treated 3T3-L1 adipocytes for 24h elevated IL-6 levels; activated the NF-κB pathway cascade; increased protein expression of IL-6R, TLR-4, MYD88, and TRAF6; and increased the nuclear activity of NF-κB (p50 and p65) DNA binding. Adiponectin and IL-10 inhibited the elevation of IL-6 levels and activated NF-κB (p50 and p65) DNA binding. Taken together, the present results provide evidence that adiponectin and IL-10 have an important role in the anti-inflammatory response in adipocytes. In addition, inhibition of NF-κB signaling pathways may be an excellent strategy for the treatment of inflammation in obese individuals.