UV-irradiation-induced DNA immobilization and functional utilization of DNA on nonwoven cellulose fabric

• Published in: Biomaterials Vol. 22; no. 23; pp. 3121 - 3126
• Main Authors: Yamada, Masanori, Kato, Kozue, Shindo, Kazuna, Nomizu, Motoyoshi, Haruki, Masahiro, Sakairi, Nobuo, Ohkawa, Kousaku, Yamamoto, Hiroyuki, Nishi, Norio
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.12.2001; Elsevier Science
• Subjects: Antibacterial activity; Biological and medical sciences; Cellulose; DNA; Endocrine disruptor; Endocrine Glands - drug effects; Escherichia coli - growth & development; Functional biomaterial; Immobilized DNA; Intercalation; Medical sciences; Polychlorinated Biphenyls - pharmacology; Staphylococcus aureus - growth & development; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Ultraviolet Rays; UV irradiation; Antibacterial activity; UV irradiation; Endocrine disruptor; Intercalation; Functional biomaterial; Immobilized DNA; Escherichia coli; Immobilization; Bioactive material; Experimental study; Functional polymer; Intercalation compound; Infection; Ultraviolet radiation; DNA; Cellulose derivatives; Bacteria; Micrococcales; Biomaterial; Micrococcaceae; Antibacterial agent; Bibliographic review; Staphylococcus aureus; Enterobacteriaceae; Biomedical engineering
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(01)00061-8

Immobilization of double-stranded DNA onto nonwoven cellulose fabric by UV irradiation and utilization of DNA-immobilized cloth were examined. The immobilized DNA was found to be stable in water, with the maximum amount of fabric-immobilized DNA being approximately 20 mg/g of nonwoven fabric. The DNA-immobilized cloth could effectively accumulate endocrine disruptors and harmful DNA intercalating pollutants, such as dibenzo- p-dioxin, dibenzofuran, biphenyl, benzo[ a]pyrene and ethidium bromide. Additionally, DNA-immobilized cloth was found to bind metal ions, such as Ag +, Cu 2+, and Zn 2+. The maximum amounts of bound Ag +, Cu 2+, and Zn 2+ onto DNA-immobilized cloth (1 g) were approximately 5, 2, and 1 mg, respectively. DNA-immobilized cloth containing Ag + showed antibacterial activity against Escherichia coli and Staphylococcus aureus. DNA-immobilized cloth without metal ion and with Cu 2+ or Zn 2+ did not show antibacterial activity. These results suggest that immobilized DNA imparts useful functionality to cloth. DNA-immobilized cloth prepared by UV irradiation has potential to serve as a useful biomaterial for medical, engineering, and environmental application.