Whole Genome Analysis and Assessment of the Metabolic Potential of Gordonia rubripertincta Strain 112, a Degrader of Aromatic and Aliphatic Compounds

• Published in: Biology (Basel, Switzerland) Vol. 12; no. 5; p. 721
• Main Authors: Frantsuzova, Ekaterina, Bogun, Alexander, Solomentsev, Viktor, Vetrova, Anna, Streletskii, Rostislav, Solyanikova, Inna, Delegan, Yanina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.05.2023; MDPI
• Subjects: alkanes; Aromatic compounds; biodegradation; Catechol; Communication; Cytochrome P450; Ecosystems; genome assembly; Genomes; Gordonia; Gordonia rubripertincta; Hexadecane; Hydrocarbons; Laboratories; Metabolic pathways; Metabolism; Microorganisms; Molecular modelling; Nucleotides; Pollutants; Soil microorganisms; Soil sciences; Strains (organisms); Variance analysis; United States > US; Russia; catechol; aromatic compounds; biodegradation; alkanes; Gordonia rubripertincta; genome assembly
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology12050721

The application of strains in biotechnologies of environmental purification as degraders of pollutants of different chemical structures is an interesting research topic. The strain 112 (IEGM112) is capable of utilizing diesel fuel, alkanes, and aromatic compounds. The aim of this work was to study the potential of 112 as a degrader of aromatic and aliphatic compounds and analyze its complete genome in comparison with other known strains. The genome had a total length of 5.28 Mb and contained 4861 genes in total, of which 4799 were coding sequences (CDS). The genome contained 62 RNA genes in total, of which 50 were tRNAs, three were ncRNAs, and nine were rRNAs. The strain bears plasmid elements with a total length of 189,570 nucleotides (plasmid p1517). The strain can utilize 10.79 ± 1.17% of hexadecane and 16.14 ± 0.16% of decane over 3 days of cultivation. In the genome of the strain, we have found metabolic pathways of alkane (cytochrome P450 hydroxylases) and catechol (ortho- and meta-pathways) degradation. These results will help us to further approach the fundamental study of the processes occurring in the strain cells and to enrich our knowledge of the catabolic capabilities of .