Construction of novel shuttle vectors and a cosmid vector for the glutamic acid-producing bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum

• Published in: Gene Vol. 39; no. 2; pp. 281 - 286
• Main Authors: Kiyoshi, Miwa, Kazuhiko, Matsui, Mahito, Terabe, Koichi, Ito, Masaaki, Ishida, Hiroshi, Takagi, Shigeru, Nakamori, Konosuke, Sano
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 1985; Amsterdam Elsevier; New York, NY
• Subjects: bacteriophage flA; Biological and medical sciences; Biotechnology; Brevibacterium - genetics; Chromosome Mapping; cohesive end; Corynebacterium - genetics; Cosmids; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Genetic Vectors; Glutamates - metabolism; Methods. Procedures. Technologies; pBR325; Plasmids; pUB110; Recombinant DNA; transduction; Transduction, Genetic; Vectors (cloning, transfer, expression). Insertion sequences and transposons; Km; SDS; transduction; Cm; Recombinant DNA; cohesive end; Ap; Tc; pBR325; R; cfu; PEG; S; u; kb; pUB110; TEN; bacteriophage flA; pfu; Glutamic acid; Bactériophage f1; Cosmid; Brevibacteriaceae; Genetic transfer; Virus; Brevibacterium lactofermentum; Aminoacid; Corynebacterium glutamicum; Shuttle vector; Bacteria; Actinomycetes; Corynebacteriaceae; Transduction; Molecular cloning; Vector
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(85)90324-5

Novel cloning vectors for glutamic acid-producing bacteria have been constructed. Two cryptic plasmids, pAM330 from Brevibacterium lactofermentum and pHM1519 from Corynebacterium glutamicum, were used as precursors, and recombined with pBR325 or pUB110. Resultant composite plasmids were able to propagate and to express the Cm R or Km R phenotype in B. lactofermentum and C. glutamicum. A smaller, high-copy-number plasmid, pAJ43, was also isolated following deletion of a part of the pAM330-pBR325 composite plasmid. Furthermore, a cosmid vector, which can be packaged and transduced through phage infection, has been developed using a cohesive-end fragment of the f 1A phage and plasmid pAJ43. These plasmids are suitable for use as cloning vectors in the glutamic acid-producing bacteria.