Quantitative determination of uric acid using CdTe nanoparticles as fluorescence probes

• Published in: Biosensors & bioelectronics Vol. 77; pp. 359 - 365
• Main Authors: Jin, Dongri, Seo, Min-Ho, Huy, Bui The, Pham, Quoc-Thai, Conte, Maxwell L., Thangadurai, Daniel, Lee, Yong-Ill
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.03.2016
• Subjects: Biotechnology; Cadmium Compounds - chemistry; Cadmium tellurides; Capping; Enzyme; Fluorescence; Fluorescence quenching; Fluorescent Dyes - chemical synthesis; Humans; Hydrogen peroxide; Ligands; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Nanoparticles; Peroxidase - chemistry; Quenching; Spectrometry, Fluorescence - methods; Tellurium - chemistry; Urate Oxidase - chemistry; Uric acid; Uric Acid - urine; Nanoparticles; Hydrogen peroxide; Uric acid; Enzyme; Fluorescence quenching
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.09.057

A convenient enzymatic optical method for uric acid detection was developed based on the fluorescence quenching of ligand-capped CdTe nanoparticles by H2O2 which was generated from the enzymatic reaction of uric acid. The interactions between the CdTe nanoparticles capped with different ligands (glutathione, 3-mercaptopropionic acid, and thioglycerol) and H2O2 were investigated. The fluorescence quenching studies of GSH-capped CdTe nanoparticles demonstrated an excellent sensitivity to H2O2. The effects of uric acid, uricase and H2O2 on the fluorescence intensity of CdTe nanoparticles were also explored. The detection conditions, reaction time, pH value, incubation period and the concentration of uricase and uric acid were optimized. The detection limit of uric acid was found to be 0.10µM and the linear range was 0.22–6µM under the optimized experimental conditions. These results typify that CdTe nanoparticles could be used as a fluorescent probe for uric acid detection. •Fluorescence intensity of CdTe NPs has been quenched efficiently by H2O2.•A simple optical sensing for uric acid based on enzymatic uricase/peroxidase system.•The technique shows high sensitivity, accuracy, and rapid for detecting uric acid.•The proposed technique is capable for the detection uric acid in urine samples.