Geraniol synthase whose mRNA is induced by host-selective ACT-toxin in the ACT-toxin-insensitive rough lemon (Citrus jambhiri)

• Published in: Journal of plant physiology Vol. 169; no. 14; pp. 1401 - 1407
• Main Authors: Shishido, Hodaka, Miyamoto, Yoko, Ozawa, Rika, Taniguchi, Shiduku, Takabayashi, Junji, Akimitsu, Kazuya, Gomi, Kenji
• Format: Journal Article
• Language: English
• Published: Munich Elsevier GmbH 15.09.2012; Elsevier
• Subjects: Alternaria - chemistry; Alternaria alternata; Amino Acid Sequence; Biological and medical sciences; Citrus; Citrus - drug effects; Citrus - enzymology; Citrus - genetics; Citrus - immunology; DNA, Complementary - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Genes, Plant - genetics; Geraniol; Green Fluorescent Proteins - metabolism; Host-Parasite Interactions - drug effects; Host-Parasite Interactions - genetics; Host-selective toxin; Molecular Sequence Data; Mycotoxins - toxicity; Phosphoric Monoester Hydrolases - chemistry; Phosphoric Monoester Hydrolases - genetics; Phosphoric Monoester Hydrolases - metabolism; Plant Leaves - drug effects; Plant Leaves - genetics; Plant physiology and development; Protein Transport - drug effects; Reproducibility of Results; RNA, Messenger - genetics; RNA, Messenger - metabolism; Subcellular Fractions - drug effects; Subcellular Fractions - metabolism; Terpene synthase; Citrus; GC–MS; ORF; Terpene synthase; TPS; HST; Host-selective toxin; Geraniol; Enzyme; Citrus fruit; Rutaceae; Host; Selectivity; Synthase; Toxin; Terpene; Messenger RNA; Plant physiology; Dicotyledones; Angiospermae; Spermatophyta
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2012.05.003

Host-selective toxins (HSTs) produced by some strains of Alternaria alternata are selectively toxic to certain cultivars of plants. However, the role of HSTs in toxin-insensitive plants is currently unknown. Here, we studied the role of ACT-toxin using an ACT-toxin producing A. alternata strain SH20 and the ACT-toxin-insensitive plant rough lemon. Induction of some defense related genes in response to SH20 were faster or stronger than in response to the ACT-toxin deficient SH20 mutant. By sequencing subtractive PCR clones obtained from mRNA of rough lemon leaves inoculated with SH20 after subtraction with that of the ACT-toxin deficient SH20 mutant, we isolated the SH20-responsive genes in rough lemon. Among the SH20-responsive genes analyzed in this study, we isolated a terpene synthase (TPS) gene, RlemTPS3. We also determined that RlemTPS3 localizes to the chloroplast and produces the monoterpene geraniol.