Production of multifunctional lipases by Penicillium verrucosum and Penicillium brevicompactum under solid state fermentation of babassu cake and castor meal

• Published in: Bioprocess and biosystems engineering Vol. 34; no. 2; pp. 145 - 152
• Main Authors: Silva, Marceli Fernandes, Freire, Denise M. G, de Castro, Aline Machado, Di Luccio, Marco, Mazutti, Marcio A, Oliveira, J. Vladimir, Treichel, Helen, Oliveira, Débora
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.02.2011; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Babassu cake; babassu oil cake; Biological and medical sciences; Biotechnology; carboxylic ester hydrolases; castor bean meal; Chemistry; Chemistry and Materials Science; Culture Media - chemistry; Environmental Engineering/Biotechnology; esterification; Esterification activity; Fermentation; Food Science; Fundamental and applied biological sciences. Psychology; Fungal Proteins - biosynthesis; Hydrolytic activity; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Lipase - biosynthesis; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Original Paper; Penicillium - enzymology; Penicillium - growth & development; Penicillium brevicompactum; Penicillium verrucosum; Raw materials; solid state fermentation; Solid state physics; triacylglycerol lipase; Babassu cake; Hydrolytic activity; Lipase; Castor meal; Esterification activity; Solid state fermentation; Enzyme; Triacylglycerol lipase; Esterases; Lipases; Esterification; Carboxylic ester hydrolases; Fungi; Hydrolysis; Penicillium brevicompactum; Production; Hydrolases; Solid substrate fermentation; Fungi Imperfecti
• ISSN: 1615-7591; 1615-7605; 1615-7605
• DOI: 10.1007/s00449-010-0455-1

The main objective of this work was to optimize lipase production, in terms of hydrolytic and esterification activities, by Penicillium brevicompactum and Penicillium verrucosum in solid state fermentation using agroindustrial residues as raw material. Maxima hydrolytic activities of 48.6 and 87.7 U/g were achieved when P. brevicompactum was cultured in babassu cake and castor meal, respectively. Higher esterification activities (around 244 U/g) were achieved when P. brevicompactum was used as microorganism and babassu cake as raw material. Different experimental conditions led to these promising values, clearly showing that no correlation can be attributed between hydrolytic and esterification activities. In spite of the several applications of lipases which are capable of catalyze synthesis reactions, only few works in this subject are presented in the literature, especially when low cost raw materials are used.