The Impact of Chronic Stress on Behavior and Body Mass in New Animal Models

• Published in: Brain sciences Vol. 13; no. 10; p. 1492
• Main Authors: Alexa, Anisia Iuliana, Zamfir, Carmen Lăcrămioara, Bogdănici, Camelia Margareta, Oancea, Andra, Maștaleru, Alexandra, Abdulan, Irina Mihaela, Brănișteanu, Daniel Constantin, Ciobîcă, Alin, Balmuș, Miruna, Stratulat-Alexa, Teodora, Ciuntu, Roxana Elena, Severin, Florentina, Mocanu, Mădălina, Leon, Maria Magdalena
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.10.2023; MDPI
• Subjects: Analysis; Animal models; Animals; Anxiety; Behavior; Body mass; Circadian rhythm; Cognitive ability; Fitness equipment; Humidity; Light; light exposure variation; open field; Open-field behavior; Physiological aspects; Physiology; Psychological aspects; R&D; Research & development; Running; Stress; Stress (Psychology); swimming; Temperature; Romania
• ISSN: 2076-3425; 2076-3425
• DOI: 10.3390/brainsci13101492

(1) Background: Exposure to different sources of stress can have a significant effect on both psychological and physical processes. (2) Methods: The study took place over a period of 34 days and included a total of 40 animals. Regarding the exposure to chronic stressors, we opted for physiological, non-invasive stressors, e.g., running, swimming, and changes in the intensity of light. An unforeseen stress batch was also created that alternated all these stress factors. The animals were divided into five experimental groups, each consisting of eight individuals. In the context of conducting the open field test for behavioral assessment before and after stress exposure, we aimed to investigate the impact of stress exposure on the affective traits of the animals. We also monitored body mass every two days. (3) Results: The control group exhibited an average increase in weight of approximately 30%. The groups exposed to stress factors showed slower growth rates, the lowest being the running group, recording a rate of 20.55%, and the unpredictable stress group at 24.02%. The anxious behavior intensified in the group with unforeseen stress, in the one with light variations, and in the running group. (4) Conclusions: Our research validates the animal model of intermittent light exposure during the dark phase as a novel method of inducing stress. The modification of some anxiety parameters was observed; they vary according to the type of stress. Body mass was found to increase in all groups, especially in the sedentary groups, likely due to the absence of cognitive, spatial, and social stimuli except for cohabitation.