Solid phase microextraction as a methodology in the detection of irradiation markers in ground beef

• Published in: Food chemistry Vol. 110; no. 2; pp. 531 - 537
• Main Authors: Caja, M.M., Ruiz del Castillo, M.L., Blanch, G.P.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 15.09.2008; [Amsterdam]: Elsevier Science; Elsevier
• Subjects: 1,3-Bis(1,1-dimethylethyl)benzene; 2-Dodecylcyclobutanone; Biological and medical sciences; detection; food analysis; Food industries; food irradiation; Fundamental and applied biological sciences. Psychology; General aspects; Ground beef; Irradiation; irradiation markers; Meat and meat product industries; Methods of analysis, processing and quality control, regulation, standards; microextraction; new methods; solid phase extraction; solid phase microextraction; SPME; Irradiation; 1,3-Bis(1,1-dimethylethyl)benzene; 2-Dodecylcyclobutanone; SPME; Ground beef; Methodology; Meat product; Marker; Palmitic acid; Minced meat; Solid phase microextraction; Irradiated product; Analysis method; Saturated fatty acid; Benzene; l,3-Bis(l,l-dimethylethyl)benzene; Control method; Beef; Detection
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2008.02.024

The usefulness of solid phase microextraction (SPME) to detect the occurrence of the irradiation markers 2-dodecylcyclobutanone (2-DCB) and 1,3-bis(1,1-dimethylethyl)benzene in irradiated ground beef was evaluated. To that aim, beef samples were irradiated with different irradiation doses and subsequently examined together with non-irradiated beef samples used as control samples. The SPME conditions applied were selected as a result of performing an optimization process including different fibers (PDMS, DVB/CAR/PDMS, polyacrylate and PDMS/DVB), as well as extraction times (10, 25 and 40min) and temperatures (40 and 60°C). For comparison, 2-DCB and 1,3-bis(1,1-dimethylethyl)benzene were additionally identified in some of the samples by steam distillation–solvent extraction (SDE). Although this study is a preliminary work, from the results obtained SPME seemed to be a rapid and valuable technique to determine 2-DCB and 1,3-bis(1,1-dimethylethyl)benzene in ground beef subjected to irradiation, offering advantages over other methods reported in the literature. In addition, SPME allowed to confirm the validity of 2-DCB as an useful marker to distinguish non-irradiated from irradiated ground beef. On the contrary, the occurrence of 1,3-bis(1,1-dimethylethyl)benzene was however established in both types of samples by SPME and SDE.