Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes

Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1996; p. 199
Main Authors Mallela, Shamroop Kumar, Patel, Devang Maheshkumar, Ducasa, Gloria Michelle, Merscher, Sandra, Fornoni, Alessia, Al-Ali, Hassan
Format Journal Article
LanguageEnglish
Published United States 01.01.2019
Subjects
Cell Culture Techniques - methods
Cell Differentiation
Cell Line
Fluorescent Dyes - chemistry
Humans
Image Processing, Computer-Assisted - methods
Intravital Microscopy - methods
Lipid Droplets - metabolism
Lipid Metabolism
Lipidomics - methods
Microscopy, Confocal - methods
Podocytes - cytology
Podocytes - metabolism
Software
Human podocytes
Phenotypic drug discovery
Cell-based assay
Lipid droplets
High content screening
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Summary:Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging and quantifying LDs have become essential tools for detecting changes in cellular lipid metabolism, which could be an important indicator of disease onset or progression. We previously reported on the accumulation of LDs in podocytes of the kidney glomerulus in nephrological diseases of metabolic and non-metabolic origin. Here, we describe a high-content analysis (HCA) method for automated detection and quantification of LDs in differentiated human podocytes.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9488-5_17