Kitasatospora putterlickiae sp. nov., isolated from rhizosphere soil, transfer of Streptomyces kifunensis to the genus Kitasatospora as Kitasatospora kifunensis comb. nov., and emended description of Streptomyces aureofaciens Duggar 1948

• Published in: International journal of systematic and evolutionary microbiology Vol. 53; no. 6; pp. 2033 - 2040
• Main Authors: Groth, Ingrid, Schutze, Barbara, Boettcher, Theresa, Pullen, Christian B, Rodriguez, Carlos, Leistner, Eckhard, Goodfellow, Michael
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.2003; Society for General Microbiology
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - physiology; Bacterial plant pathogens; Bacteriology; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Kitasatospora kifunensis; Kitasatospora putterlickiae; Microbiology; Miscellaneous; Molecular Sequence Data; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Soil Microbiology; Species Specificity; Streptomyces - classification; Systematics; Actinomycetales; Rhizosphere; Soils; Streptomycetaceae; Bacteria; Actinomycetes; Streptomyces aureofaciens
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.02674-0

1 Hans-Knöll-Institut für Naturstoff-Forschung e.V., D-07745 Jena, Germany 2 Rheinische Friedrich-Wilhelms-Universität, Institut für Pharmazeutische Biologie, D-53115 Bonn, Germany 3 School of Biology, King George VI Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK Correspondence Michael Goodfellow m.goodfellow{at}ncl.ac.uk A polyphasic study was undertaken to establish the taxonomic position of a rhizosphere isolate that had been assigned provisionally to the genus Kitasatospora . The organism, isolate F18-98 T , was found to have chemical and morphological properties that were consistent with its classification as a Kitasatospora strain. Direct 16S rDNA sequence data confirmed the taxonomic position of the strain, following the generation of phylogenetic trees by using three treeing algorithms. The organism formed a 16S rDNA subclade with Kitasatospora azatica and Streptomyces kifunensis , but was distinguished readily from the latter by using a combination of biochemical and physiological properties. Genotypic and phenotypic data show that strain F18-98 T should be classified in the genus Kitasatospora as a novel species, for which the name Kitasatospora putterlickiae sp. nov. is proposed; the type strain has been deposited in culture collections as strain F18-98 T (=DSM 44665 T =NCIMB 13932 T ). It is also proposed that Streptomyces kifunensis should be transferred to the genus Kitasatospora as Kitasatospora kifunensis comb. nov. An emended description of Streptomyces aureofaciens Duggar 1948 is also given. Abbreviations: A 2 pm, diaminopimelic acid; ISP, International Streptomyces Project The GenBank/EMBL/DDBJ accession numbers for the 16S rDNA sequences of Kitasatospora putterlickiae F18-98 T and Streptomyces aureofaciens IMET 43577 T are AY189976 and AY289116 , respectively. The accession number for the 16S–23S rDNA spacer region of Kitasatospora putterlickiae is AY189977 .