Metabolomic identification of potential phospholipid biomarkers for chronic glomerulonephritis by using high performance liquid chromatography–mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 860; no. 1; pp. 134 - 140
• Main Authors: Jia, Lewen, Wang, Chang, Zhao, Sumin, Lu, Xin, Xu, Guowang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2007; Elsevier Science
• Subjects: Adult; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Biomarkers - blood; Chromatography, High Pressure Liquid - methods; Chronic Disease; Chronic glomerulonephritis; Female; Fundamental and applied biological sciences. Psychology; General pharmacology; Glomerulonephritis - blood; Glomerulonephritis - metabolism; High performance liquid chromatography; Humans; Kidney Failure, Chronic - blood; Male; Mass spectrometry; Mass Spectrometry - methods; Medical sciences; Middle Aged; Pharmacology. Drug treatments; Phospholipids; Phospholipids - blood; Phospholipids - metabolism; Phospholipids; Mass spectrometry; Chronic glomerulonephritis; High performance liquid chromatography; Kidney disease; Glomerulonephritis; Urinary system disease; HPLC chromatography; Biological marker; Phospholipid; Complex lipid; Chronic; Metabonomics; Qualitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2007.10.033

Plasma phospholipids metabolic profile of chronic glomerulonephritis was investigated using high performance liquid chromatography/mass spectrometry (LC/MS) and principal component analysis. The plasma samples of 18 patients with chronic glomerulonephritis, 17 patients with chronic renal failure (CRF) without renal replacement therapy and 18 healthy persons were collected and analyzed. It was found that combination of the LC/MS technique with PCA can be successfully applied to phospholipid profile analysis. The results showed that primary chronic glomerulonephritis and CRF had phospholipids metabolic abnormality. Nineteen phospholipid species were identified as possible biomarkers in plasma samples of chronic glomerulonephritis and chronic renal failure. Moreover, the identification of the molecular structure of the potential phospholipid markers was obtained by ESI-MS/MS experiment. It suggests that phospholipids can be used as potential biomarkers on the progress of primary chronic glomerulonephritis.