An in vitro approach to evaluate the nutraceutical value of plant foliage against Haemonchus contortus
Nutraceutical plants provide nutrients for the animal as well as secondary compounds that can affect the biology and survival of gastrointestinal nematodes (GIN). Current screening of nutraceutical plants is based on in vitro evidence of anthelmintic (AH) activity against different life stages of GI...
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Published in | Parasitology research (1987) Vol. 117; no. 12; pp. 3979 - 3991 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.12.2018
Springer Springer Nature B.V Springer Verlag (Germany) |
Subjects | |
Online Access | Get full text |
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Summary: | Nutraceutical plants provide nutrients for the animal as well as secondary compounds that can affect the biology and survival of gastrointestinal nematodes (GIN). Current screening of nutraceutical plants is based on in vitro evidence of anthelmintic (AH) activity against different life stages of GIN, but nutritional information is omitted or scarce. This study proposes an integral in vitro screening protocol to identify the nutraceutical value of the foliage from plant species consumed by small ruminants, using
Haemonchus contortus
as a biological model. The leaves from
Acacia collinsii
,
A
.
pennatula
,
Bunchosia swartziana
,
Gymnopodium floribundum
,
Havardia albicans
,
Leucaena leucocephala
,
Lysiloma latisiliquum
,
Mimosa bahamensis
,
Piscidia piscipula
, and
Senegalia gaumeri
were evaluated for their chemical composition and in vitro digestibility. Acetone:water extracts (70:30) from leaves of each plant were evaluated using the egg hatch assay and larval exsheathment inhibition assay. Respective effective concentrations 50% (EC
50
) were determined for each assay. The ten plant species showed good nutritional value for ruminants, including crude protein (> 10%), metabolizable energy (> 2.9 MJ/kg DM), and varied CT content (from 1.0 to 37.6%). The best AH activity against
H
.
contortus
eggs (EC
50
= 401.8 μg/mL) and L
3
(EC
50
= 83.1 μg/mL) was observed for
S
.
gaumeri
extract. Although all the plant species showed in vitro nutraceutical potential, the leaves of
S
.
gaumeri
had the best values. The proposed in vitro protocol showed to be useful for the integral assessment of the nutraceutical potential of different plant species as it included the nutritional value and the AH activity against eggs and L
3
in the selected plant species. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0932-0113 1432-1955 |
DOI: | 10.1007/s00436-018-6107-0 |