Serum and glucocorticoid kinase 1 promoted the growth and migration of non-small cell lung cancer cells

• Published in: Gene Vol. 576; no. 1; pp. 339 - 346
• Main Authors: Xiaobo, Yu, Qiang, Lin, Xiong, Qin, Zheng, Ruan, Jianhua, Zhou, Zhifeng, Lin, Yijiang, Su, Zheng, Jian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2016
• Subjects: Animals; Base Sequence; beta Catenin - genetics; beta Catenin - metabolism; Beta-catenin/TCF signaling; blood serum; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Cell growth and migration; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; gene expression regulation; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; genes; growth retardation; Humans; Immediate-Early Proteins - genetics; Immediate-Early Proteins - metabolism; lung neoplasms; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; metastasis; Mice, Nude; Molecular Sequence Data; neoplasm cells; NSCLC; phosphorylation; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; SGK1; Signal Transduction; TCF Transcription Factors - metabolism; transcription (genetics); Xenograft Model Antitumor Assays; GFP; PBS; NSCLC; PI3K; FBS; Beta-catenin/TCF signaling; SGK1; ATCC; Cell growth and migration
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2015.10.072

Serum and glucocorticoid kinase 1 (SGK1) has been reported to be up-regulated in non-small cell lung cancer (NSCLC). However, its functions in NSCLC remained unclear. Here, SGK1 was found to be up-regulated in NSCLC samples. Over-expression of SGK1 promoted the growth and migration of NSCLC cells, while down-regulation of SGK1 inhibited the growth, migration and metastasis of NSCLC cells. SGK1 promoted the phosphorylation of GSK3 beta and the accumulation of beta-catenin, up-regulation of the target genes downstream of beta-catenin/TCF signaling, and activating the transcriptional activity of beta-catenin/TCF complex. Collectively, SGK1 might promote the progression of NSCLC through activating beta-catenin/TCF signaling. •SGK1 is overexpressed in NSCLC samples.•SGK1 positively regulates the growth, migration and metastasis of NSCLC cells.•SGK1 activates beta-catenin/TCF signaling in NSCLC cells.