Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions

• Published in: Applied microbiology and biotechnology Vol. 63; no. 2; pp. 143 - 146
• Main Authors: CHEN, X, ZHANG, D.-J, QI, W.-T, GAO, S.-J, XIU, Z.-L, XU, P
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.12.2003; Springer Nature B.V
• Subjects: Aerobic conditions; Aerobiosis; Anaerobic conditions; Anaerobiosis; Bacteria; Biological and medical sciences; Bioreactors; Biotechnology; Biotechnology - methods; Culture Media; Ethanol; Fermentation; Fermentation - drug effects; Fundamental and applied biological sciences. Psychology; Glycerol; Glycerol - metabolism; Klebsiella pneumoniae; Klebsiella pneumoniae - growth & development; Klebsiella pneumoniae - metabolism; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Productivity; Propylene Glycols - metabolism; Temperature; Production; Microorganism culture; Bacteria; Aerobe; Fermentation; Propanediol; Semicontinuous; Klebsiella pneumoniae; Enterobacteriaceae
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-003-1369-5

The microbial production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under micro-aerobic conditions was investigated in this study. The experimental results of batch fermentation showed that the final concentration and yield of 1,3-PD on glycerol under micro-aerobic conditions approached values achieved under anaerobic conditions. However, less ethanol was produced under microaerobic than anaerobic conditions at the end of fermentation. The batch micro-aerobic fermentation time was markedly shorter than that of anaerobic fermentation. This led to an increment of productivity of 1,3-PD. For instance, the concentration, molar yield, and productivity of 1,3-PD of batch micro-aerobic fermentation by K. pneumoniae DSM 2026 were 17.65 g/l, 56.13%, and 2.94 g l(-1) h(-1), respectively, with a fermentation time of 6 h and an initial glycerol concentration of 40 g/l. Compared with DSM 2026, the microbial growth of K. pneumoniae AS 1.1736 was slow and the concentration of 1,3-PD was low under the same conditions. Furthermore, the microbial growth in fed-batch fermentation by K. pneumoniae DSM 2026 was faster under micro-aerobic than anaerobic conditions. The concentration, molar yield, and productivity of 1,3-PD in fed-batch fermentation under micro-aerobic conditions were 59.50 g/l, 51.75%, and 1.57 g l(-1) h(-1), respectively. The volumetric productivity of 1,3-PD under microaerobic conditions was almost twice that of anaerobic fed-batch fermentation, at 1.57 and 0.80 g l(-1) h(-1), respectively.