Inositol hexakisphosphate receptor identified as the clathrin assembly protein AP-2

• Published in: Biochemical and biophysical research communications Vol. 187; no. 1; pp. 158 - 163
• Main Authors: Voglmaier, Susan M., Keen, James H., Murphy, Jo-Ellen, Ferris, Christopher D., Prestwich, Glenn D., Snyder, Solomon H., Theibert, Anne B.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 31.08.1992; Elsevier
• Subjects: Adaptor Proteins, Vesicular Transport; Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Brain - ultrastructure; Cattle; Cell receptors; Cell structures and functions; Coated Pits, Cell-Membrane - chemistry; DNA - chemistry; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Mice; Miscellaneous; Molecular and cellular biology; Molecular Sequence Data; Peptide Fragments - chemistry; Phosphoproteins - chemistry; Polymerase Chain Reaction; Rats; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - genetics; Receptors, Cytoplasmic and Nuclear; Sequence Homology, Nucleic Acid; IP 4; IP 6R; ACN; TFA; PCR; CHAPS; IP 6; Vertebrata; Mammalia; Complementary DNA; Nucleotide sequence; Homology; Inositol phosphate; Aminoacid sequence; Biological receptor
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(05)81473-1

To clarify the function of the receptor binding protein for inositol hexakisphosphate (IP 6), we obtained a partial amino acid sequence from the purified protein and a partial nucleotide sequence from a cDNA clone of the gene. The sequences are essentially identical to those of the α-subunit of the clathrin assembly protein AP-2. The IP 6 receptor protein analyzed by SDS-PAGE contains a series of subunits which are the same as those of AP-2. Antibodies to AP-2 react with the IP 6 receptor protein in immunoblot analysis.