Distinctive Under-Expression Profile of Inflammatory and Redox Genes in the Blood of Elderly Patients with Cardiovascular Disease

• Published in: Journal of inflammation research Vol. 14; pp. 429 - 442
• Main Authors: Milanesi, Elena, Manda, Gina, Dobre, Maria, Codrici, Elena, Neagoe, Ionela V, Popescu, Bogdan O, Bajenaru, Ovidiu Alexandru, Spiru, Luiza, Tudose, Catalina, Prada, Gabriel I, Davidescu, Eugenia I, Piñol-Ripoll, Gerard, Cuadrado, Antonio
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 2021; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: Aged patients; aging-related diseases; AKT1 protein; Blood; Canada; Cardiovascular disease; Cardiovascular diseases; CD40 antigen; Chemokines; Comorbidity; Cytokines; Development and progression; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes therapy; Diseases; Dyslipidemia; Gene expression; Gene regulation; Genes; Genetic transcription; Growth factors; Hypercholesterolemia; Hypertension; Inflammation; Interferon regulatory factor 1; Investigations; Leukocytes; Medical research; Medicine, Experimental; Metabolism; Morbidity; NF-κB protein; nf-κb signaling; Older people; Original Research; Oxidative stress; Physiological aspects; Prognosis; Proteins; redox metabolism; RelA protein; RelB protein; Romania; Senescence; Spain; Stat1 protein; Statistical analysis; TRAF2 protein; Transcription; Type 2 diabetes; Canada; Romania; Spain; NF-κB signaling; aging-related diseases; redox metabolism; cardiovascular disease; inflammation; oxidative stress
• ISSN: 1178-7031; 1178-7031
• DOI: 10.2147/JIR.S280328

Chronic low-grade inflammation and oxidative stress are present in most of the pathologic mechanisms underlying non-communicable diseases. Inflammation and redox biomarkers might therefore have a value in disease prognosis and therapy response. In this context, we performed a case-control study for assessing in whole blood the expression profile of inflammation and redox-related genes in elderly subjects with various comorbidities. In the blood of 130 elderly subjects with various pathologies (cardiovascular disease, hypertension, dyslipidemia including hypercholesterolemia, type 2 diabetes mellitus), kept under control by polyvalent disease-specific medication, we investigated by pathway-focused qRT-PCR a panel comprising 84 inflammation-related and 84 redox-related genes. The study highlights a distinctive expression profile of genes critically involved in NF-κB-mediated inflammation and redox signaling in the blood of patients with cardiovascular disease, characterized by significant down-regulation of the genes and . This gene expression profile defines the transcriptional status of blood leukocytes in stable disease under medication control, without discriminating between disease- and therapy-related changes. The study brings preliminary proof on a minimally invasive strategy for monitoring disease in patients with cardiovascular pathology, from the point of view of inflammation or redox dysregulation in whole blood.