Liquid chromatography-mass spectrometry and related techniques for purity assessment in early drug discovery

• Published in: Journal of separation science Vol. 28; no. 14; pp. 1742 - 1750
• Main Authors: Molina-Martin, Manuel, Marin, Aranzazu, Rivera-Sagredo, Alfonso, Espada, Alfonso
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.09.2005; WILEY‐VCH Verlag; Wiley
• Subjects: Analysis; Biological and medical sciences; Caffeine - chemistry; Caffeine - isolation & purification; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Early drug discovery; General pharmacology; History, 20th Century; History, 21st Century; Mass Spectrometry - instrumentation; Mass Spectrometry - methods; Medical sciences; Orthogonal techniques; Peak capacity; Pharmaceutical Preparations - chemistry; Pharmaceutical Preparations - history; Pharmaceutical Preparations - isolation & purification; Pharmacology. Drug treatments; Purity assessment; Quantitative purity; Drug; Peak resolution; Chemical analysis; Coupled method; HPLC chromatography; Purity control; Chemiluminescence; Quantitative purity; Orthogonal techniques; Purity assessment; Research and development; Load capacity; Ultraviolet detector; Plate efficiency; Evaporative light scattering detector; Mass spectrometry; Peak capacity; Quantitative analysis; Early drug discovery
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.200500107

The combination of HPLC and MS has become the most valuable analytical tool to determine the identity and purity of a drug substance in the drug discovery arena over the past decade. This article describes different LC/MS configurations and their broad applicability to meet the fundamental analytical requirements involved in discovering new drugs. In addition, the value of chemiluminescence nitrogen detection for absolute purity determination and the convenience of CE as an orthogonal separation technique to HPLC are also discussed. In summary, LC/MS‐based methodologies that implicate automation, various levels of throughput and open access systems have proved to be an integral part of the drug discovery process. As a result, the paradigm of high‐quality/‐quantity information is fulfilled in a timely fashion.