Determination of mismatched donor HLA in kidney transplant recipients with unknown donor HLA phenotypes

• Published in: Clinical transplantation Vol. 24; no. 5; pp. E178 - E181
• Main Authors: Kwok, Janette, Chan, Gavin S. W., Lam, M. F., Yan, Tony, Tang, Lydia, Kwong, K. M., Chan, K. W., Chan, T. M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2010
• Subjects: Antibodies; Biopsy; DNA - analysis; DNA - genetics; Donors; Feasibility Studies; Graft rejection; Graft Rejection - genetics; Graft Rejection - immunology; Histocompatibility antigen HLA; Histocompatibility Testing; HLA Antigens - genetics; HLA Antigens - immunology; human leukocyte antigen (HLA) typing; Humans; Immunophenotyping; kidney transplant; Kidney transplantation; Kidney Transplantation - immunology; Living Donors; mismatched human leukocyte antigens; Nucleotide sequence; Oligonucleotides; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Primers; Proteinuria; Tissue typing; Transplantation, Homologous
• ISSN: 0902-0063; 1399-0012
• DOI: 10.1111/j.1399-0012.2010.01246.x

Kwok J, Chan GSW, Lam MF, Yan T, Tang L, Kwong KM, Chan KW, Chan TM. Determination of mismatched donor HLA in kidney transplant recipients with unknown donor HLA phenotypes.
Clin Transplant 2010 DOI: 10.1111/j.1399‐0012.2010.01246.x 
© 2010 John Wiley & Sons A/S. :  Objective:  To determine donor human leukocyte antigen (HLA) from renal allograft biopsies in transplant recipients whose donor HLA phenotype is not known. Methods:  Renal allograft biopsies were obtained from seven renal transplant recipients when indicated for allograft dysfunction or proteinuria. DNA was extracted fresh from allograft specimens, and HLA typing was performed with polymerase chain reaction‐specific sequence primers (PCR‐SSP) and polymerase chain reaction‐sequence‐specific oligonucleotides (PCR‐SSO). Results:  HLA typing of the seven renal allograft biopsies was composed of both recipient and donor HLA phenotypes, allowing the determination of the donor HLA and the degree of HLA mismatching. Conclusions:  Deducing mismatched donor HLA antigens in renal allograft recipients enables detection of donor‐specific antibodies, and the management of humoral rejection, and enables more appropriate selection of a donor organ should future retransplantation be required.