Establishment and characterization of an in vivo model for Epstein-Barr virus positive gastric carcinoma

• Published in: Journal of medical virology Vol. 79; no. 9; pp. 1343 - 1348
• Main Authors: Oh, Sang Taek, Cha, Jung-Ho, Shin, Dong-Jik, Yoon, Sungjoo Kim, Lee, Suk Kyeong
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2007; Wiley-Liss
• Subjects: Animals; Biological and medical sciences; Cell Line, Tumor; Epstein-Barr virus; Epstein-Barr Virus Infections - genetics; Epstein-Barr Virus Infections - virology; Fundamental and applied biological sciences. Psychology; gastric carcinoma; Genotype; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - physiology; Human viral diseases; in vivo model; Infectious diseases; Medical sciences; Mice; Mice, Nude; Microbiology; Miscellaneous; Stomach Neoplasms - genetics; Stomach Neoplasms - virology; tumorigenesis; Viral diseases; Virology; Virus Latency; Gammaherpesvirinae; Stomach; Carcinoma; Herpesviridae; Epstein Barr virus; Malignant tumor; Virus; In vivo; in vivo model; Models; Epstein-Barr virus; tumorigenesis; gastric carcinoma
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.20876

Research regarding the role of the Epstein–Barr virus (EBV) in gastric carcinogenesis has been hampered by the absence of a suitable model system. SNU‐719 is a gastric carcinoma cell line naturally infected with EBV. This cell line developed tumors in nude mice approximately 40–56 days after inoculation. SNU‐719 also showed low serum dependency and anchorage independent growth in vitro. The developed tumors expressed EBERs, EBNA1, and LMP2A but not other EBV latent genes. Additionally, Qp was active and either mono‐ or bi‐clonal EBV genome was observed in the tumor tissues. Because the developed tumors retained characteristics of EBV‐associated gastric cancer, this cell line could serve as a useful in vivo system to investigate the tumorigenesis mechanism and treatment methods for this type of tumor. J. Med. Virol. 79:1343–1348, 2007. © Wiley‐Liss, Inc.