Impaired overload-induced muscle growth is associated with diminished translational signalling in aged rat fast-twitch skeletal muscle

• Published in: The Journal of physiology Vol. 574; no. 1; pp. 291 - 305
• Main Authors: Thomson, David M., Gordon, Scott E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK The Physiological Society 01.07.2006; Blackwell Publishing Ltd; Blackwell Science Inc
• Subjects: Adaptation, Physiological; Aging - metabolism; Animals; Cumulative Trauma Disorders - metabolism; Muscle Fibers, Fast-Twitch - metabolism; Muscle Proteins - metabolism; Muscle, Skeletal - physiopathology; Protein Modification, Translational; Rats; Rats, Inbred F344; Signal Transduction; Skeletal Muscle and Exercise
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/jphysiol.2006.107490

Impaired overload-induced protein synthesis and growth in aged fast-twitch skeletal muscle may result from diminished responsiveness of signalling intermediates controlling protein translation. Yet, potential age-related signalling decrements have never been examined in direct parallel with impaired overload-induced muscle growth in any model. To this end, we used Western blotting to examine the contents and phosphorylation states of mammalian target of rapamycin (mTOR) and its downstream translational signalling intermediates, 70 kDa ribosomal protein S6 kinase (S6k), ribosomal protein S6 (rpS6), eukaryotic elongation factor 2 (eEF2), and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1), in conjunction with impaired growth in 1 week overloaded fast-twitch plantaris muscles (via unilateral gastrocnemius ablation) of old (O; 30 months) versus young adult (YA; 8 months) male Fischer344 × Brown Norway rats. The significantly ( P ≤ 0.05) diminished growth (assessed by total muscle protein content) in overloaded O muscles (5.6 ± 1.7 versus 19.3 ± 2.9% in YA) was accompanied by significant impairments in the phosphorylation states of mTOR (Ser 2448 ), S6k (impaired at the mTOR-specific Thr 389 residue but not at Thr 421 /Ser 424 ), rpS6 (Ser 235/236 ) and 4E-BP1 (gel shift), as well as deficits in total eEF2 accretion. Moreover, in overloaded muscles across both age groups, phospho-S6k at Thr 389 (but not at Thr 421 /Ser 424 ), 4E-BP1 phosphorylation status, and total eEF2 accretion were all positively correlated with percentage muscle hypertrophy, and negatively correlated with the phosphorylation (Thr 172 ) of 5′-AMP-activated protein kinase (AMPK; which inhibits translational signalling and protein synthesis in young muscle at rest). As previously published by ourselves, AMPK was hyperphosphorylated in O versus YA muscles used in the current investigation. The present results provide solid evidence that impaired overload-induced growth in aged fast-twitch muscle may partly result from multiple-level decrements in signalling pathway(s) controlling protein translation, and also provide an initial indication that AMPK hyperactivation with age may potentially lie upstream of these decrements.