The DNA polymerase-encoding gene of African swine fever virus: sequence and transcriptional analysis

• Published in: Gene Vol. 136; no. 1; pp. 103 - 110
• Main Authors: Rodríguez, Javier M., Yáñez, Rafael J., Rodríguez, JoséF., Viñuela, Eladio, Salas, María L.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 22.12.1993; Amsterdam Elsevier; New York, NY
• Subjects: ADN; AFRICAN SWINE FEVER VIRUS; African Swine Fever Virus - enzymology; African Swine Fever Virus - genetics; Amino Acid Sequence; aphidicolin; Base Sequence; Biological and medical sciences; Conserved Sequence; DNA; DNA replication; DNA, Viral; DNA-Directed DNA Polymerase - genetics; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE; GENE EXPRESSION; GENES; Genes, Viral; Genetics; Microbiology; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; Open Reading Frames; SECUENCIA NUCLEICA; Sequence Analysis, DNA; sequence homology; Sequence Homology, Amino Acid; SEQUENCE NUCLEIQUE; transcription initiation; Transcription, Genetic; Virology; VIRUS DE LA PESTE PORCINA AFRICANA; VIRUS PESTE PORCINE AFRICAINE; ORF; transcription initiation; nt; HSV1; dNTP; α-human; bp; tsp; S; ASF; kb; oligo; PolI; ASFV; aa; DNA replication; aphidicolin; GCG; δ-yeast; G1207R; sequence homology; ϵ-yeast; Exo; gene expression; pfu; Nucleotide sequence; Transcription; Enzyme; Transferases; Sequence alignment; Gene organization; African swine fever virus; Virus; Nucleotidyltransferases; Iridoviridae; DNA-directed DNA polymerase; Aminoacid sequence; Structural analysis
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(93)90453-A

The putative DNA polymerase-encoding gene of African swine fever virus has been sequenced. The gene, designated G1207R, is located in the central region of the viral genome, and encodes a protein of 1207 amino acids (aa) with a predicted M r of 139 835. The gene is transcribed at both early and late stages of infection into a 4.1-kb RNA. Transcription is initiated at tsp, 8 nucleotides (nt) upstream from the start codon. Open reading frame (ORF) G1207R contains four direct repeats in tandem close to the 3'-end. Each repeat consists of 12 nt, coding for the reiterated sequence, K/NPAG. The deduced aa sequence of G1207R shows significant similarity with DNA polymerases from cellular and viral origin, belonging to the α-like family of DNA polymerases. In particular, the G1207R protein presents a colinear arrangement of all the 3'→5' exonuclease and polymerization highly conserved aa regions characteristic of this group of DNA-dependent DNA polymerases.