Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae

Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a ( ) from was identified and characterized. The consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric...

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Published inInsects (Basel, Switzerland) Vol. 11; no. 5; p. 281
Main Authors Xu, Huihui, Hao, Zhongping, Wang, Lifang, Li, Shuangjiao, Guo, Yuruo, Dang, Xiangli
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 05.05.2020
MDPI
Subjects
Amino acids
Binding sites
Cloning
Deoxyribonucleic acid
development
DNA
expression patterns
Fungi
Gene expression
Hemocytes
immunity
Immunology
Insecticides
Insects
Iron
Isaria cicadae
Larvae
Level (quantity)
Metabolism
Microorganisms
Molecular weight
Nodulation
Pathogens
Peptides
Pest control
Plutella xylostella
Proteins
Pupation
RNA-mediated interference
Transcription
Transferrin
Transferrins
Beijing China
United States > US
China
Plutella xylostella
RNAi
development
transferrin
immunity
Isaria cicadae
expression patterns
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Abstract Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a ( ) from was identified and characterized. The consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. transcript level was highest in fat bodies among various tissues. The transcript levels increased significantly after the stimulation of pathogens. A decrease in expression via RNA interference enhanced the susceptibility of to the fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in immunity against fungal infection and insect development.
AbstractList Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a Trf (PxTrf) from Plutella xylostella was identified and characterized. The PxTrf consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although PxTrf was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. PxTrf transcript level was highest in fat bodies among various tissues. The PxTrf transcript levels increased significantly after the stimulation of pathogens. A decrease in PxTrf expression via RNA interference enhanced the susceptibility of P. xylostella to the Isaria cicadae fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in P. xylostella immunity against fungal infection and insect development.
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a Trf ( PxTrf ) from Plutella xylostella was identified and characterized. The PxTrf consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although PxTrf was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. PxTrf transcript level was highest in fat bodies among various tissues. The PxTrf transcript levels increased significantly after the stimulation of pathogens. A decrease in PxTrf expression via RNA interference enhanced the susceptibility of P. xylostella to the Isaria cicadae fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in P. xylostella immunity against fungal infection and insect development.
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a ( ) from was identified and characterized. The consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae. transcript level was highest in fat bodies among various tissues. The transcript levels increased significantly after the stimulation of pathogens. A decrease in expression via RNA interference enhanced the susceptibility of to the fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in immunity against fungal infection and insect development.
Author Li, Shuangjiao
Hao, Zhongping
Wang, Lifang
Guo, Yuruo
Xu, Huihui
Dang, Xiangli
AuthorAffiliation 3 School of Horticulture, Anhui Agricultural University, Hefei 230036, China; wlf2000@ahau.edu.cn
2 Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China; hzp5187@sina.com
1 Key Laboratory of Biology and Green Control of Plant Diseases and Insect Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; xh15755396709@163.com (H.X.); lsj0926@sohu.com (S.L.); guoyurrrrrrruo@outlook.com (Y.G.)
AuthorAffiliation_xml – name: 3 School of Horticulture, Anhui Agricultural University, Hefei 230036, China; wlf2000@ahau.edu.cn
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– name: 2 Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China; hzp5187@sina.com
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/32380643$$D View this record in MEDLINE/PubMed
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Issue 5
Keywords Plutella xylostella
RNAi
development
transferrin
immunity
Isaria cicadae
expression patterns
Language English
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Snippet Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a ( ) from was identified and characterized. The...
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a Trf (PxTrf) from Plutella xylostella was...
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a Trf ( PxTrf ) from Plutella xylostella was...
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StartPage 281
SubjectTerms Amino acids
Binding sites
Cloning
Deoxyribonucleic acid
development
DNA
expression patterns
Fungi
Gene expression
Hemocytes
immunity
Immunology
Insecticides
Insects
Iron
Isaria cicadae
Larvae
Level (quantity)
Metabolism
Microorganisms
Molecular weight
Nodulation
Pathogens
Peptides
Pest control
Plutella xylostella
Proteins
Pupation
RNA-mediated interference
Transcription
Transferrin
Transferrins
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Title Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae
URI https://www.ncbi.nlm.nih.gov/pubmed/32380643
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