Suppression of Transferrin Expression Enhances the Susceptibility of Plutella xylostella to Isaria cicadae
Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a ( ) from was identified and characterized. The consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric...
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Published in | Insects (Basel, Switzerland) Vol. 11; no. 5; p. 281 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
05.05.2020
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Transferrins (Trfs) are multifunctional proteins with key functions in iron transport. In the present study, a
(
) from
was identified and characterized. The
consisted of a 2046-bp open reading frame, which encoded a 681 amino acid protein with a molecular weight of 73.43 kDa and had an isoelectric point of 7.18. Only a single iron domain was predicted in the N-lobe of PxTrf. Although
was expressed ubiquitously, the highest levels of expression were observed in the fourth instar larvae.
transcript level was highest in fat bodies among various tissues. The
transcript levels increased significantly after the stimulation of pathogens. A decrease in
expression via RNA interference enhanced the susceptibility of
to the
fungus and inhibited hemocyte nodulation in response to the fungal challenge. In addition, a considerable increase in the pupation rate was observed in larvae treated with double-stranded PxTrf (dsPxTrf). Overall, according to the results, PxTrf may participate in
immunity against fungal infection and insect development. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
ISSN: | 2075-4450 2075-4450 |
DOI: | 10.3390/insects11050281 |