Cloning of the murine unconventional myosin gene Myo9b and identification of alternative splicing

• Published in: Gene Vol. 240; no. 2; pp. 389 - 398
• Main Authors: Grewal, Prabhjit K., Jones, Anne-Marie, Maconochie, Mark, Lemmers, Richard J.F., Frants, Rune R., Hewitt, Jane E.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 29.11.1999
• Subjects: 3' Untranslated Regions - genetics; Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; C57Bl/6; cDNA cloning; Cloning, Molecular; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Ear, Inner - embryology; Ear, Inner - metabolism; Expression; Gene Expression Regulation, Developmental; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Inbred Strains; Molecular Sequence Data; Muridae; Myo9b gene; Myosins - genetics; myr5 gene; Polymorphism; Polymorphism, Genetic; Protein Isoforms - genetics; RNA - genetics; RNA - metabolism; RT–PCR; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tissue Distribution; kb, kilobase; g, guanosine; dNTPs, deoxyribonucleoside triphosphates; RT–PCR; UTR, untranslated region; EST, expressed sequence tag; C57Bl/6; cDNA cloning; c, cytidine; t, thymidine; Expression; a, adenosine; bp, base pair; RT–PCR, reverse transcriptase polymerase chain reaction; aa, amino acid; Polymorphism

We report the cloning of a cDNA for the mouse unconventional myosin Myo9b, the orthologue of the rat myr5 and human MYOIXb genes. A full-length spleen cDNA of 7087 bp encoding a protein of 1961 amino acids was isolated. By RT–PCR, we show that Myo9b is expressed in a wide range of tissues, including...