Cloning of the murine unconventional myosin gene Myo9b and identification of alternative splicing
We report the cloning of a cDNA for the mouse unconventional myosin Myo9b, the orthologue of the rat myr5 and human MYOIXb genes. A full-length spleen cDNA of 7087 bp encoding a protein of 1961 amino acids was isolated. By RT–PCR, we show that Myo9b is expressed in a wide range of tissues, including...
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Published in | Gene Vol. 240; no. 2; pp. 389 - 398 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
29.11.1999
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Subjects | |
Online Access | Get full text |
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Summary: | We report the cloning of a cDNA for the mouse unconventional myosin
Myo9b, the orthologue of the rat
myr5 and human
MYOIXb genes. A full-length spleen cDNA of 7087
bp encoding a protein of 1961 amino acids was isolated. By RT–PCR, we show that
Myo9b is expressed in a wide range of tissues, including heart, brain, muscle and inner ear. In addition, we have identified two alternatively spliced exons. Equivalent exons have not been previously reported for either the human or rat homologues. These exons are located in the
Myo9b specific actin-binding site insert of the head domain and in the tail region. A third splice form utilizing an alternative reading frame within the 3′
UTR is also described. Several polymorphisms within the coding region were identified; of interest is an in-frame 33
bp imperfect duplication within the tail region that was observed only in the C57Bl/6 strain.
Myo9b has been previously mapped to mouse chromosome 8 and is a candidate for the mouse mutations myodystrophy and quinky. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(99)00459-X |