Phosphatidylinositol 4-phosphate 5-kinase Iγ_v6, a new splice variant found in rodents and humans

• Published in: Biochemical and biophysical research communications Vol. 411; no. 2; pp. 416 - 420
• Main Authors: Xia, Yang, Irvine, Robin F., Giudici, Maria-Luisa
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 29.07.2011; Academic Press
• Subjects: Alternative Splicing; Amino Acid Sequence; Animals; brain; Cercopithecus aethiops; COS Cells; exons; Humans; Isoenzymes - chemistry; Isoenzymes - genetics; Lipid kinase; Mice; Molecular Sequence Data; nucleotides; Phosphatidylinositol 4,5-bisphosphate; Phosphatidylinositol 4-phosphate 5-kinase; Phosphotransferases (Alcohol Group Acceptor) - chemistry; Phosphotransferases (Alcohol Group Acceptor) - genetics; PIP2; Rats; Splice variant; PIP5KI; Lipid kinase; Splice variant; Phosphatidylinositol 4-phosphate 5-kinase; Phosphatidylinositol 4,5-bisphosphate; PIP2
• ISSN: 0006-291X; 1090-2104; 1090-2104
• DOI: 10.1016/j.bbrc.2011.06.168

► In this study we investigate splice variation in the lipid kinase PI4P 5-kinase Iγ. ► We report a new splice variant in rodents. ► We show that this new variant has a wide tissue distribution. ► We show, by identifying a new human splice site, that humans also express this variant. ► Finally, we show that another known splice variant, hitherto thought to be present only in rodents, is also found in humans. Phosphatidylinositol 4-phosphate 5-kinase Iγ (PIP5KIγ) is subject to extensive C-terminal splice variation, with four variants, PIP5KIγ_v1, 2, 4 and 5, described in humans Schill and Anderson (2009) [7]. Here firstly, we report a new rodent splice variant, which includes the exon that was previously unique to the rodent neuron-specific PIP5KIγ93 Giudici et al. (2006) [6], but which omits the C-terminal exon of PIP5KIγ93; this new variant shows a wide tissue expression pattern in mouse. Secondly, we show that in humans there is an alternative splicing site 78 nucleotides from the start of exon 16c, such that humans additionally express both PIP5KIγ93 (which we now call PIP5KIγ_v3) specifically in brain and, again expressed more widely, the new variant described here, which we now name PIP5KIγ_v6.