Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt

• Published in: International journal of systematic and evolutionary microbiology Vol. 54; no. 1; pp. 247 - 252
• Main Authors: Hozzein, Wael N, Li, Wen-Jun, Ali, Mohammed Ibrahim A, Hammouda, Ola, Mousa, Ahmed S, Xu, Li-Hua, Jiang, Cheng-Lin
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.2004; Society for General Microbiology
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Actinomycetales - physiology; Bacterial plant pathogens; Bacteriology; Biological and medical sciences; Desert Climate; DNA, Ribosomal - genetics; Egypt; Fundamental and applied biological sciences. Psychology; Microbiology; Microscopy, Electron, Scanning; Miscellaneous; Molecular Sequence Data; Nocardiopsis alkaliphila; Phospholipids - metabolism; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; RNA, Ribosomal, 16S - genetics; Systematics; Africa; Egypt; Egypt, Arab Rep; Actinomycetales; Soils; Alkalophily; Nocardiopsis; Bacteria; Nocardiaceae; Actinomycetes
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.02832-0

1 Botany Department, Faculty of Science, Cairo University, Beni-Suef, Egypt 2 Key Laboratory for Microbial Resources of the Ministry of Education, Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, P.R. China Correspondence Cheng-Lin Jiang lihxu{at}ynu.edu.cn An alkaliphilic actinomycete strain, designated YIM 80379 T , was isolated from a soil sample collected from the eastern desert of Egypt and subjected to polyphasic taxonomy. The strain produced substrate and aerial mycelia on different media, with an optimum pH for growth of 9·5–10 and scarce or no growth at pH 7. Strain YIM 80379 T contained meso -diaminopimelic acid, no diagnostic sugars, type PIII phospholipids and MK-10(H 6 ) and MK-10(H 8 ) as the predominant menaquinones. All of these characters assign isolate YIM 80379 T consistently to the genus Nocardiopsis . This was confirmed by 16S rDNA analysis. It can be differentiated from all Nocardiopsis species with validly published names by phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization results. On the basis of polyphasic evidence, a novel species, Nocardiopsis alkaliphila sp. nov., is proposed. The type strain of the species is YIM 80379 T (=CCTCC AA001031 T =DSM 44657 T ). Abbreviations: DPG, diphosphatidylglycerol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PIM, phosphatidylinositol mannosides; PME, phosphatidyl methylethanolamine Published online ahead of print on 1 August 2003 as DOI 10.1099/ijs.0.02832-0. The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of strain YIM 80379 T is AY230848 .