Host Immunosuppression Induced by Steinernema feltiae , an Entomopathogenic Nematode, through Inhibition of Eicosanoid Biosynthesis

• Published in: Insects (Basel, Switzerland) Vol. 11; no. 1; p. 33
• Main Authors: Chandra Roy, Miltan, Lee, Dongwoon, Kim, Yonggyun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.12.2019; MDPI
• Subjects: Antibiotics; Arachidonic acid; Bacteria; Biosynthesis; Butterflies & moths; Dexamethasone; Diet; eicosanoids; Entomopathogenic nematodes; Fatty acids; Gene expression; Hemocytes; Hemolymph; Immune response; Immune response (cell-mediated); Immunosuppression; Immunosuppressive agents; Infections; Insects; Kanamycin; Larvae; Mortality; Nematodes; Pathogenicity; Pathogens; Phenoloxidase; Phenols; Phospholipase A2; Physical characteristics; pla2; plutella xylostella; Septicemia; Steinernema feltiae; Virulence; United States > US; Steinernema feltiae; PLA2; Plutella xylostella; eicosanoids; immunosuppression
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects11010033

K1 (Filipjev) (Nematode: Steinernematidae), an entomopathogenic nematode, was isolated and identified based on its morphological and molecular diagnostic characteristics. Its infective juveniles (IJs) were highly pathogenic to three lepidopteran (LC = 23.7-25.0 IJs/larva) and one coleopteran (LC = 39.3 IJs/larva) insect species. Infected larvae of the diamondback moth, (L.) (Insecta: Lepidoptera), exhibited significant reduction in phospholipase A (PLA ) activity in their plasma. The decrease of PLA activity was followed by significant septicemia of the larvae infected with . Insecticidal activity induced by was explained by significant immunosuppression in cellular immune responses measured by hemocyte nodule formation and total hemocyte count (THC). Although infection suppressed nodule formation and THC in the larvae, an addition of arachidonic acid (AA, a catalytic product of PLA ) rescued these larvae from fatal immunosuppression. In contrast, an addition of dexamethasone (a specific PLA inhibitor) enhanced the nematode's pathogenicity in a dose-dependent manner. To discriminate the immunosuppressive activity of a symbiotic bacterium ( (Proteobacteria: Enterobacterales)) from the nematode, kanamycin was applied to after nematode infection. It significantly inhibited the bacterial growth in the hemolymph. Compared to nematode treatment alone, the addition of antibiotics to nematode infection partially rescued the immunosuppression measured by phenol oxidase activity. Consequently, treatment with antibiotics significantly rescued the larvae from the insecticidal activity of . These results suggest that immunosuppression induced by infection of depends on its symbiotic bacteria by inhibiting eicosanoid biosynthesis, resulting in significant insect mortality. However, the addition of antibiotics or AA could not completely rescue the virulence of the nematode, suggesting that the nematode itself also plays a role in its insecticidal activity.