SCI1 Is a Direct Target of AGAMOUS and WUSCHEL and Is Specifically Expressed in the Floral Meristematic Cells

The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that th...

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Published inFrontiers in plant science Vol. 12; p. 642879
Main Authors Cruz, Joelma O, San Martin, Juca A B, Lubini, Greice, Strini, Edward J, Sobral, Rómulo, Pinoti, Vitor F, Ferreira, Pedro B, Thomé, Vanessa, Quiapim, Andréa C, Dornelas, Marcelo C, Pranchevicius, Maria Cristina S, Madueño, Francisco, Costa, M Manuela R, Goldman, Maria Helena S
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 18.03.2021
Subjects
co-expression
floral determinacy
flower development
meristematic cells
Nicotiana tabacum
Plant Science
transcriptional control
co-expression
flower development
meristematic cells
transcriptional control
floral determinacy
Nicotiana tabacum
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Summary:The specified floral meristem will develop a pre-established number of floral organs and, thus, terminate the floral meristematic cells. The floral meristematic pool of cells is controlled, among some others, by WUSCHEL (WUS) and AGAMOUS (AG) transcription factors (TFs). Here, we demonstrate that the ( ) gene, a cell proliferation regulator, starts to be expressed since the floral meristem specification of and is expressed in all floral meristematic cells. Its expression is higher in the floral meristem and the organs being specified, and then it decreases from outside to inside whorls when the organs are differentiating. is co-expressed with ( ) in the floral meristem and the whorl primordia at very early developmental stages. Later in development, is co-expressed with ( ) in the floral meristem and specialized tissues of the pistil. analyses identified -regulatory elements for these TFs in the genomic sequence. Yeast one-hybrid and electrophoresis mobility shift assay demonstrated that both TFs interact with the promoter sequence. Additionally, the luciferase activity assay showed that NAG1 clearly activates expression, while NtWUS could not do so. Taken together, our results suggest that during floral development, the spatiotemporal regulation of by NtWUS and NAG1 may result in the maintenance or termination of proliferative cells in the floral meristem, respectively.
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Edited by: Stefan de Folter, UGA-Langebio, Center for Research and Advanced Studies (CINVESTAV), Mexico
Reviewed by: Toshiro Ito, Nara Institute of Science and Technology (NAIST), Japan; Veronica Gregis, University of Milan, Italy
Present address: Juca A. B. San Martin, Academia Nacional de Ciencias Exactas, Físicas y Naturales, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Botánica Darwinion, San Isidro, Argentina
This article was submitted to Plant Development and EvoDevo, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2021.642879