Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins

Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are de...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 1258; p. 209
Main Authors Nettleship, Joanne E, Watson, Peter J, Rahman-Huq, Nahid, Fairall, Louise, Posner, Mareike G, Upadhyay, Abhishek, Reddivari, Yamini, Chamberlain, Jonathan M G, Kolstoe, Simon E, Bagby, Stefan, Schwabe, John W R, Owens, Raymond J
Format Journal Article
LanguageEnglish
Published United States 01.01.2015
Subjects
Animals
Escherichia coli - genetics
Escherichia coli - metabolism
HEK293 Cells - metabolism
Humans
Mammals - genetics
Mammals - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Transfection - methods
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Summary:Transient transfection of human embryonic kidney cells (HEK 293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for the expression and purification of both secreted and intracellular proteins using transient expression in HEK 293 cells are described.
ISSN:1940-6029
DOI:10.1007/978-1-4939-2205-5_11