Bcl - xL Promotes the Survival of Motor Neurons Derived from Neural Stem Cells

• Published in: Biology (Basel, Switzerland) Vol. 12; no. 1; p. 132
• Main Authors: Wu, Yunqin, Peng, Xiaohua, Ang, Song, Gao, Yue, Chi, Yue, Wang, Jinling, Tang, Chengcheng, Zhou, Xiaoqing, Feng, Yanxian, Zhang, Kun, Zou, Qingjian, Chen, Min
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.01.2023; MDPI
• Subjects: Amyotrophic lateral sclerosis; Antibodies; Apoptosis; Bcl-x protein; Bcl-xL; Brief Report; Cell culture; Disease; Doxycycline; Ectopic expression; Genomes; Islet-1 protein; Motor neuron diseases; motor neurons; Neural stem cells; Neurodegenerative diseases; Neurons; Neurotoxicity; Spinal cord injuries; Stem cell transplantation; Stem cells; survival; United States > US; motor neurons; Bcl-xL; neural stem cells; survival
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology12010132

Neural stem cell (NSC) transplantation creates new hope for the treatment of neurodegenerative disorders by direct differentiation into neurons. However, this technique is limited by poor survival and functional neuron deficiency. In this research study, we generated pro-survival murine NSCs (mNSCs) via the ectopic expression of - . A doxycycline (Dox)-inducible system was also integrated into the mNSC genome. The four gene-modified mNSCs can rapidly and effectively differentiate into motor neurons after Dox treatments. Ectopic - could resist replating-induced stress, glutamate toxicity, neuronal apoptosis and remarkably promote the survival of motor neurons. Taken together, we established genetically modified mNSCs with improved survival, which may be useful for motor neuron degenerative diseases.