High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor

• Published in: Biosensors & bioelectronics Vol. 22; no. 8; pp. 1593 - 1599
• Main Authors: Kim, Namsoo, Park, In-Seon, Kim, Dong-Kyung
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.03.2007; Elsevier Science
• Subjects: AChE immobilization; Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Carbofuran - analysis; Chemical Precipitation; Enzymes, Immobilized; Fundamental and applied biological sciences. Psychology; High-sensitivity detection; Insecticides - analysis; Methods. Procedures. Technologies; Model pesticides; Organophosphorus Compounds - analysis; QCM-precipitation sensor; Quartz; Sensitivity and Specificity; Various methods and equipments; QCM-precipitation sensor; Model pesticides; High-sensitivity detection; AChE immobilization; Enzyme; Pesticides; Immobilization; Esterases; Acetylcholinesterase; Carboxylic ester hydrolases; Precipitation; Sensitivity; Biosensor; Hydrolases; Organophosphorus compounds; Detection; Carbamate; Quartz microbalance
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2006.07.009

The effect of acetylcholinesterase (AChE) immobilization over the surface of a quartz crystal microbalance (QCM), by chemisorption of the AChE thiolated with a heterobifunctional cross-linker, sulfo-succinimidyl-6-[3-(2-pyridyldithio)propionamido]hexanoate, and carboxyl-amine coupling of AChE to 3-mercaptopropionic acid self-assembled monolayer, on the responses of a batch-type QCM-precipitation sensor was compared, resulting in a better sensitivity and binding efficiency in the former method. When an inhibition study with the developed sensor was undertaken at the optimized AChE immobilization with varying concentrations of a model organophosphorus pesticide EPN and carbamate one carbofuran, a sensitive detection for them was possible with the limit of detection corresponding to 1.55 × 10 −8 and 1.30 × 10 −9 M, respectively.